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POT7-RFP

Plasmid

#65323

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT6-RFP

Plasmid

#65322

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pT7_circRAJ31_C873U

Plasmid

#69932

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

pT7_circRAJ31_C873U

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCri-15b

Plasmid

#61336

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Yeast

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSTC1

Plasmid

#39241

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pEB602

Plasmid

#22071

Depositor

Emmanuelle Bouveret

Article

Gully et al Proteomics. 2006 Jan . 6(1):282-93.

Type

Empty backbone

Tags

CBP

Expression

Bacterial

Available Since

Oct. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pSEVA47_Helper

Plasmid

#231420

Purpose

M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ296.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

gp1-gp11

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-iPASnFR-L

Plasmid

#225236

Purpose

AAV expression of iPASnFR-L from hSyn promoter

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Use

AAV

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-iPASnFR-L

Plasmid

#225235

Purpose

AAV expression of iPASnFR-L from GFAP promoter

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Use

AAV

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLCKOv3

Plasmid

#209046

Purpose

Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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GPI-Gamillus

Plasmid

#197914

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus

Expression

Mammalian

Mutation

none

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SYN-L-sDarken

Plasmid

#184798

Purpose

Low affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

L-sDarken

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEY13

Plasmid

#191019

Purpose

Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

arrd-4 (ZK938.2 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMCSGC-GB1

Plasmid

#186786

Purpose

E. coli expression vector containing N-terminal 6His-GB1-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYCTK034

Plasmid

#176738

Purpose

The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Saccharomyces cerevisiae (barrier part).

Depositor

Victor Sourjik

Article

Krink et al bioRxiv 2021

Insert

bar1Sc

Use

Synthetic Biology

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mEos2-NS

Plasmid

#115549

Purpose

Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Lifeact-mEos2

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL294

Plasmid

#167834

Purpose

Encodes OptoLAC1 circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (LAA)

Expression

Bacterial

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL296

Plasmid

#167835

Purpose

Encodes OptoLAC2 circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (AAV)

Expression

Bacterial

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-NS

Plasmid

#140874

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 5' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway Cloning

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pPAP002

Plasmid

#153489

Purpose

Level 1 episomal expression plasmid for P. pastoris

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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