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pLL3.7-EF-EYFP-YAP1_WT-PolyA

Plasmid

#112284

Purpose

Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

WT human YAP1-2gamma

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-mTrim21ΔRING-Box

Plasmid

#105517

Purpose

Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of RING domain (aa124-462)

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCLIPf-H2B Control Plasmid

Plasmid

#101134

Purpose

Expression of CLIP-tagged Histone H2B in mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Histone H2B (H2BC11 Human)

Tags

CLIP-tag (CLIPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

Plasmid

#89608

Purpose

Expression of Luciferase in human cells. 3rd generation lentiviral vector

Depositor

Fernando Fierro

Article

Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

Insert

Luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Nup35

Plasmid

#87342

Purpose

To express human Nup35 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

nucleoporin 35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-TPC2

Plasmid

#80153

Purpose

Intracellular Ca2+ store marker

Depositor

Santiago Di Pietro

Article

Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

Insert

TPCN2 (TPCN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Caveolin-3

Plasmid

#68396

Purpose

Fluorescently tagged Caveolin-3 for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsNot3

Plasmid

#37372

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot3 (CNOT3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Gateway Cloning

Tags

HA and Myr

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
EGFP-Ahi1

Plasmid

#30494

Depositor

Joseph Gleeson

Article

Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

Insert

Ahi1 (AHI1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERalpha (ESR1 Human)

Expression

Yeast

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
CAGGS-losSTOPlox-ClaI flp-in

Plasmid

#21548

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

Use

Mouse Targeting

Expression

Mammalian

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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hTLR1 flag

Plasmid

#13081

Depositor

Ruslan Medzhitov

Insert

TLR1 (TLR1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-18 have been replaced with mammalia…

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
hCdh1 (1-125)

Plasmid

#8508

Depositor

Joan Ruderman

Article

Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

Insert

hCdh1 (1-125) (FZR1 Human)

Use

Retroviral

Tags

His

Expression

Mammalian

Mutation

Cdh1 N-terminus. Dominant Negative mutant.

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pSIN4-EF1a-LMO2-IRES-Puro

Plasmid

#61064

Purpose

lentiviral vector for LMO2

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

LMO2 (LMO2 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1alpha

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP TEM4 FL

Plasmid

#58893

Purpose

Expresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.

Depositor

Channing Der

Article

Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

Insert

TEM4 (ARHGEF17 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L722F

Promoter

CMV

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBS2E

Plasmid

#55169

Purpose

Empty vector, integration at lacA, ampr, mlsr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP VARP

Plasmid

#42312

Depositor

Thierry Galli

Article

Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

Insert

VARP (ANKRD27 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only
pPK605

Plasmid

#38148

Depositor

Patricia Kuwabara

Inserts

unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR) (unc-119 Nematode)
pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR

Expression

Worm

Promoter

pie-1 promoter and unc-119

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pH2B-LSSmOrange

Plasmid

#37133

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

histone 2B

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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