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p1071-Lenti-Single loxP

Plasmid

#217892

Purpose

Lentiviral vector, entry vector for Lentiviral Switch-OVER

Depositor

Ulrich Elling

Publication

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1-3

Plasmid

#218798

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-3

Expression

Mammalian

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-ChREBPα

Plasmid

#211754

Purpose

Cloning vector for the expression of mouse ChREBPα

Depositor

Russell Goodman

Publication

Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

Insert

MLXIPL (Mlxipl Mouse)

Use

Cloning vector

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pZG61

Plasmid

#202575

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptII

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO OM-eLIR

Plasmid

#189004

Purpose

mitophagy-inducing adaptor protein

Depositor

Atsushi Hoshino

Publication

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OM-eLIR

Tags

HA

Expression

Mammalian

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSGKP_AcsgRNA

Plasmid

#203807

Purpose

pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

Depositor

Quanjiang Ji

Publication

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AcgRNA

Use

CRISPR

Promoter

J23119

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHis-3xFLAG-CMV14-CYB5R3

Plasmid

#204551

Purpose

Expresses 3xFLAG-tagged CYB5R3 in mammalian cells

Depositor

Masaaki Komatsu

Publication

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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H_NLS 67kDa

Plasmid

#201372

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

(NLS)SV40-EGFP-6PrA

Expression

Mammalian

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ZKSCAN1_Tau7-12WT

Plasmid

#194166

Purpose

Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7.

Depositor

Stefan Stamm

Publication

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-associated protein tau 7-12 WT

Tags

3X Flag

Expression

Mammalian

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.pCry1(408).DIO.Luciferase

Plasmid

#192623

Purpose

Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

Depositor

Michael Hastings

Publication

Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

Insert

luciferase

Use

AAV

Expression

Mammalian

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_IRF3_SMAD/FHA

Plasmid

#178479

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Publication

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

IRF3_IRF3 (IRF3 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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HA-KIF5A

Plasmid

#166958

Purpose

Expresses HA-tagged KIF5A protein in pcDNA3.1

Depositor

Rosalind Segal

Publication

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCHA1.1-Rap2a-V5

Plasmid

#149509

Purpose

Stable expression of human Rap2a.

Depositor

David Sabatini

Publication

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

RAP2 (RAP2A Human)

Use

Lentiviral

Tags

V5

Mutation

Codon Optimized Sequence

Promoter

Ubc

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI042

Plasmid

#105351

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Gal4UAS & 35S min promoter

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSYC-104

Plasmid

#74792

Purpose

pCS4+-NLS-EGFP-F2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Publication

Kim et al PLoS One. 2011 . 6(4):e18556.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-EGFP-F2A-mCherry-CAAX

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pFA1

Plasmid

#46789

Purpose

To express Fun30 in trans in budding yeast

Depositor

Patrick Varga-Weisz

Publication

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Promoter

PGAL1

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8A[Q67L]

Plasmid

#24905

Depositor

Maxence Nachury

Publication

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway Cloning

Mutation

Changed Glutamine 67 to Leucine

Available since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pBS DKK3

Plasmid

#19201

Depositor

Cliff Tabin

Publication

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Dickkopf homolog 3 (DKK3 Chicken)

Expression

Bacterial

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS Olfactomedin1

Plasmid

#19225

Depositor

Cliff Tabin

Publication

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Olfactomedin 1 (OLFM1 Chicken)

Expression

Bacterial

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
PcDNA3 - FmnI Iso1b

Plasmid

#19323

Depositor

Philip Leder

Publication

Zhou et al Exp Cell Res. 2006 Apr 15. 312(7):1119-2

Insert

FmnI Iso1b (Fmn1 Mouse)

Expression

Mammalian

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only