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pPAmKate-N1

Plasmid

#34587

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Expression

Mammalian

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

CMV

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCVII-tdTomato

Plasmid

#162752

Purpose

Retroviral (MSCV) expression of td Tomato fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

tdTomato

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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LRG2.1-CyOFP1

Plasmid

#124771

Purpose

3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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FIRESFLIP-PBCS-E670

Plasmid

#228701

Purpose

Sensor cassette FIRE2, sensor in backwards orientation, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 SENSOR

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-RNF103-CHMP3

Plasmid

#140584

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA RNF103-CHMP3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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LeGO-hEµMAR-G2

Plasmid

#52633

Purpose

3rd generation lentiviral vector for enhanced eGFP expression/marking in primary B cells and B-cell lines

Depositor

Boris Fehse

Article

Mock et al Hum Gene Ther Methods. 2012 Dec;23(6):40

Insert

hEµMAR-SFFV; eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

July 16, 2014

Availability

Academic Institutions and Nonprofits only

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LRG2.1-mOrange

Plasmid

#124772

Purpose

3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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p281 TIR1_HA_flox STOP_mH11

Plasmid

#164856

Purpose

Cre-inducible TIR1 expression vector for mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Myo1b

Plasmid

#187362

Purpose

Expresses rat myosin 1b isoform b labelled with EGFP

Depositor

Evelyne Coudrier

Article

Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

Insert

myosin 1b isoform b (Myo1b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLSG (stra8)

Plasmid

#21619

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Stra8 promoter (Stra8 Mouse)

Use

Lentiviral

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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cmv-sfGFP-GB1-scAB

Plasmid

#185794

Purpose

scFv GFP compnent for SunRISER

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

sfGFP-GB1-scAB

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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LeGO-hEµMAR-iG2

Plasmid

#52635

Purpose

3rd generation lentiviral vector for enhanced transgene and eGFP-coexpression in primary B cells and B-cell lines

Depositor

Boris Fehse

Article

Mock et al Hum Gene Ther Methods. 2012 Dec;23(6):40

Insert

hEµMAR-SFFV; IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pME18S-F-R-Olfr160

Plasmid

#149366

Purpose

Expresses Olfr160 in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

Olfr160 (Olfr160 Mouse)

Tags

FLAG and rhodopsin

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pX165-eSpCas9

Plasmid

#140564

Purpose

Expresses eSpCas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

K848A K1003A R1060A

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSG22-pBAD-TetR

Plasmid

#102451

Purpose

Expresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam DP1

Plasmid

#37968

Depositor

Jacqueline Lees

Article

Moberg et al Mol Cell Biol. 1996 Apr;16(4):1436-49.

Insert

DP1 (TFDP1 Human)

Expression

Mammalian

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)

Plasmid

#211861

Purpose

Nuclear-localized E3-DART ligase in domestication vector.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

3xNLS-HA

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-miR17

Plasmid

#21113

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

miR-17 (MIR17 Human)

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3puro-mGFP-TDRD3

Plasmid

#225469

Purpose

Express mEGFP-fusion protein of TDRD3

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

TDRD3 (TDRD3 Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP2-NS

Plasmid

#80502

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP2

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

TagBFP2 (no STOP)

Use

Multisite gateway middle entry clone

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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