We narrowed to 10,108 results for: Coli
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Plasmid#192822PurposeGolden Gate compatible plasmid with mCherry and NeoDepositorInsertmCherry and Neo
ExpressionMammalianAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mKate-MKLP1
Plasmid#140570PurposeExpresses mKate tagged MKLP1DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-NLC-DHFR-SpCas9
Plasmid#124523PurposeExpresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cellsDepositorInsertNLC-DHFR-SpCas9
UseAAVTagsDestabilized domain of E.coli dihydrofolate reduc…ExpressionMammalianPromoterCbhAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-MKLP1
Plasmid#140562PurposeExpresses mCherry tagged MKLP1DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
plko-tet-on-puromycin-shTRX1-259
Plasmid#110920PurposeTo knockdown Thioredoxin-1 following doxycycline additionDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYTRW27K_7Ti1
Plasmid#177294Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and LacZDepositorInsertPsacB-sacB
UseSynthetic Biology; Yeast expression, rrn genomic …ExpressionBacterialAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW07K_0G5
Plasmid#177279Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmRDepositorInsertPaacC1-aacC1
UseSynthetic Biology; Yeast expression, tn5 genomic …ExpressionBacterialAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW08K_0C5
Plasmid#177280Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with CmRDepositorInsertPcat-cat
UseSynthetic Biology; Yeast expression, tn5 genomic …ExpressionBacterialAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW11K_0S5
Plasmid#177282Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with SmRDepositorInsertPaadA-aadA
UseSynthetic Biology; Yeast expression, tn5 genomic …ExpressionBacterialAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW20K_0Ti1
Plasmid#177289Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcRDepositorInsertPtet-tetA(C)
UseSynthetic Biology; Yeast expression, rrn genomic …ExpressionBacterialAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas-Cas12m-ΔZF
Plasmid#192276PurposeEncodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoterDepositorInsertCas12m ΔZF
UseCRISPRExpressionBacterialMutationH459A, C552APromoterPJ23108Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS97splitRBP_S2-Enz
Plasmid#183768PurposeExpresses SHIP2-Enzyme domain flanked by RBPDepositorInsertSHIP2 (INPPL1 Human)
Tags6HIS, RBP (C-terminal) HRV3C cleavage site, and R…ExpressionBacterialMutationincludes aa K414 to aa V872 onlyPromoterT7lacAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCh-623
Plasmid#92266PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cellsDepositorInsertdGW182 6W6A (gw Fly)
TagsNHAExpressionInsectMutationThe following point mutations were introduced W23…PromoterAc5Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-695
Plasmid#92263PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cellsDepositorInsertdGW182 PAM2 mut (gw Fly)
TagsNHAExpressionInsectMutationThe following point mutations were introduced: EF…PromoterAc5Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-660
Plasmid#92259PurposePlasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
TagsNHAExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterCMVAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-665
Plasmid#92254PurposePlasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
TagsGSTExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterEF1AAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha2_KanR_BastaR_Rb7_GFP
Plasmid#186427Purposeoptimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)DepositorInsertsKanR
BastaR
Rb7
eGFP
UseSynthetic Biology; Binary vector for escherichia …ExpressionPlantMutationBsaI and BsmBI sites removedPromoterCaMV 35S and PnosAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX/PD-L1 WT
Plasmid#121466PurposeExpression of GST-human PD-L1 in E. ColiDepositorAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
Tags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry
Plasmid#230043PurposePartial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.DepositorInsertlox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
UseCre/Lox; Zebrafish expressionPromoterfabp10aAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only