165,075 results

1. pET22b RNase A

   Plasmid

   #58903

   Purpose

   Bacterial expression of bovine pancreatic ribonuclease A

   Depositor

   Ronald Raines

   Article

   delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

   Insert

   RNase A (RNASE1 Bovine)
   

   Tags

   pelB sequence

   Expression

   Bacterial

   Mutation

   See Depositor Comments below

   Promoter

   T7

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE30-His-NDK

   Plasmid

   #124136

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Nucleotide diphosphate kinase (ndk Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Bxb1-LP-v2-TC

   Plasmid

   #194326

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

   Insert

   loxP-EBFP2-attP-BleoR-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-TPNOX

   Plasmid

   #87853

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   TPNOX
   

   Tags

   C-terminal FLAG tag with a linker

   Expression

   Bacterial

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. mKeima-Red-Mito-7

   Plasmid

   #56018

   Purpose

   Localization: Mitochondria, Excitation: 440, Emission: 620

   Depositor

   Michael Davidson

   Insert

   Mito (COX8A Human)
   

   Tags

   mKeima-Red

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fuw-dCas9-Dnmt3a-P2A-tagBFP

   Plasmid

   #84569

   Purpose

   Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRKH2

   Plasmid

   #71312

   Purpose

   Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

   Depositor

   Todd Klaenhammer, Rodolphe Barrangou

   Article

   O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Expression

   Bacterial

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. hCathepsin B

   Plasmid

   #11249

   Purpose

   Mammalian expression of human cathepsin B

   Depositor

   Hyeryun Choe

   Article

   Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

   Insert

   cathepsin B (CTSB Human)
   

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Available Since

   May 25, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. MEK1-GFP

   Plasmid

   #14746

   Depositor

   Rony Seger

   Article

   Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

   Insert

   MEK1 (MAP2K1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. MaLionR

    Plasmid

    #113908

    Purpose

    Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionR
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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