169,217 results
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Plasmid#207154PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only
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PD14
Plasmid#164529PurposeExpresses HA-UbDepositorInsertUbiquitin
UseLentiviralTagsHA and UbMutationWTPromoterCMVAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
BFP Homology Donor
Plasmid#149372PurposePromoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detectionDepositorInsertCDDR BFP Homology Donor
ExpressionMammalianAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
cpstFRET-Actin
Plasmid#80644PurposeForce free control for Actin-cpstFRET-ActinDepositorInsertActin, cpstFRET
ExpressionMammalianAvailable SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-CDK12_IDG-K
Plasmid#135281PurposeGateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK12 (CDK12 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso C
Plasmid#137721PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoformDepositorInsertBRD4 short isoform (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianPromoterTight TRE promoterAvailable SinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHSE401
Plasmid#62201PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoter35S and AtU6-26pAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDY118A
Plasmid#182958PurposepSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.DepositorInsertsCas9
Gam, Beta, Exo
I-scel
Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)
Plasmid#83947PurposegRNA cassette-carrying vector with natMX markerDepositorInsertgRNA targeting ADE2 gene
ExpressionYeastAvailable SinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAT220
Plasmid#134888PurposeBacterial expressionDepositorInserteGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only