We narrowed to 31,954 results for: Eng

1. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-AncMEcoGII250

   Plasmid

   #205025

   Purpose

   Expressing engineered M.EcoGII with higher thermostability

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 250
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYC01

   Plasmid

   #224586

   Purpose

   IPTG-inducible phage φX174 lysis gene E

   Depositor

   Ophelia Venturelli

   Article

   Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

   Insert

   phage φX174 lysis gene E (phiX174p08 )
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PA1lacO-1

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

   Plasmid

   #41863

   Purpose

   This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Ai9

   Plasmid

   #22799

   Purpose

   Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

   Insert

   CAG-Floxed tdTomato
   

   Use

   Mouse Targeting

   Available Since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pnos-Cas9-nos

   Plasmid

   #62208

   Purpose

   Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Expression

   Insect

   Promoter

   nanos

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. gRNA_AAVS1-T2

   Plasmid

   #41818

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pre_II_CS3_9072_v1

   Plasmid

   #126854

   Purpose

   9072 b scaffold from compatibility group 3, made by method II

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   II_CS3_9072_v1
   

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiGuide-TagRFP-2A-BSD

    Plasmid

    #167930

    Purpose

    Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge