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Flag-FOXO4

Plasmid

#153143

Purpose

Expression of Flag tagged human FOXO4 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXO4 (FOXO4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-E29-ChR2GFP2x

Plasmid

#153437

Purpose

AAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E29 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chr2-GFP-P2A-GFP

Use

AAV

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSCMV-H2B-FT-Fast

Plasmid

#157669

Purpose

Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

Depositor

Shangqin Guo

Article

Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

Insert

Histone H2B

Use

Retroviral

Tags

Fast-FT

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0

Plasmid

#153291

Purpose

Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

EFS

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEE083

Plasmid

#149279

Purpose

T-DNA encoding TRV2 complete genome with MCS

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Type

Empty backbone

Expression

Plant

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-GFAP-NLS-CasRx-NLS-FLAG

Plasmid

#154000

Purpose

Express CasRx for AAV package

Depositor

Hui Yang

Article

Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

Insert

CasRx

Use

AAV and CRISPR

Tags

FLAG

Promoter

GFAP

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

Plasmid

#132393

Purpose

Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)

Use

CRISPR and Cre/Lox

Tags

EGFP

Expression

Mammalian

Mutation

Proline 301 to Leucine (P301L)

Promoter

TREtight

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG/GFP

Plasmid

#139980

Purpose

expresses GFP under the promoter of CAG. Transgene flanked by AAV2 ITR

Depositor

William Pu

Article

Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

Insert

Green fluorescent protein

Use

AAV

Promoter

CAG

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4max-NRTH

Plasmid

#136919

Purpose

Expresses BE4max-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL-GFPAdGO2-PGK-Neo

Plasmid

#136899

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPAdGO2

Use

Lentiviral

Tags

2xNLS

Mutation

TGG>TAG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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M3D395A-M14-dCas9

Plasmid

#134780

Purpose

Expression of fusion protein M3D395A-M14-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

METTL3/METTL14 methyltransferase domain and inactive dCas9

Expression

Mammalian

Mutation

D395A

Promoter

CMV

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen_UtrCH

Plasmid

#129607

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Utrophin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro

Plasmid

#132773

Purpose

Expression of human LMNA.

Depositor

Jan Lammerding

Article

Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Flag-humanRBM47

Plasmid

#134607

Purpose

Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

Flag-humanRBM47 (RBM47 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC6A19

Plasmid

#132231

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A19 (SLC6A19 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Target-AID (pRZ762)

Plasmid

#131300

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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TBP6.7 non-targeting gRNA lambda phage

Plasmid

#132547

Purpose

expresses gRNA for TBP-based CIRTS

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

gRNA TBP-based CIRTS

Expression

Mammalian

Promoter

hU6 promoter

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-FAM208A

Plasmid

#115845

Purpose

Encodes codon optimized HA tagged FAM208A

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HA tagged FAM208A (C3orf63 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

Plasmid

#112927

Purpose

CRISPR-a reporter assay lentiviral vector (gTetO)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-SomArchon

Plasmid

#126942

Purpose

Expression of SomArchon under CaMKII promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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