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pcDNA3.1_hE2F7mg_PP7-tag

Plasmid

#73078

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-end

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Tags

5'-PP7-tag RNA

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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WT MetNIQ in pBAD

Plasmid

#118253

Purpose

Wild Type Methionine Binding Protein and Methionine ABC Transporter in pBAD Vector for Transport Activity Assay

Depositor

Douglas Rees

Article

Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

Inserts

MetN (metN E. coli)
MetI (metI E. coli)
MetQ (metQ E. coli)

Tags

10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

Expression

Bacterial

Mutation

Changed Glutamic Acid (Glu) 295 to Alanine (Ala)

Promoter

None and araBAD Promoter

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mApple-proα2(I)-G610C

Plasmid

#119841

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

mApple

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A3-VN

Plasmid

#135506

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*03:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*03:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAP1892-1

Plasmid

#105247

Purpose

human GFP11 with tagRFP 33bp downstream in Lamin A/C

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

Insertion of GFP11 at cut tagRFP 33bp downstream in Lamin A/C (LMNA Human)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G12-E

Plasmid

#25757

Purpose

Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)

Plasmid

#110761

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean-proα2(I)

Plasmid

#119836

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

mCerulean

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLINK cyclin T1 (1-280) myc (all 200) (P#575)

Plasmid

#14610

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) (all 200) (CCNT1 Human)

Tags

myc

Expression

Mammalian

Mutation

C198A, C200A, H202A, C205A. Active in 3T3 cells.

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo-TGmiR-Gb2

Plasmid

#25746

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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