We narrowed to 15,384 results for: pET
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Plasmid#49741DepositorInsertAS22_4450
UseSynthetic BiologyExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xMS2 oligo-library based
Plasmid#158200PurposeCassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of MS2 from oligo library experiment
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i1 sgRNA / hSpCas9
Plasmid#172825PurposeMammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
G1397 DddAtox-N–SaKKH-Cas9(D10A)
Plasmid#157839Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
ExpressionMammalianAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C K80D
Plasmid#184743Purposebacterial expression of human TTR mutantsDepositorAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330 _HITI donor B6
Plasmid#172847PurposeDRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRa16_3622
Plasmid#49670DepositorInsertECF16_3622
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-MYC-KPTN
Plasmid#87043Purposemammalian expression of KPTNDepositorAvailable SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
PAX6 sgRNA2
Plasmid#68465Purposetargeting PAX6 geneDepositorAvailable SinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT112_GalL_nCDA1-xBE3
Plasmid#141318PurposeExpressing base editor nCDA1-xBE3 in yeast cellsDepositorInsertnCDA1-xBE3
UseCRISPRExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVRa12_807
Plasmid#49663DepositorInsertECF12_807
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3_Z
Plasmid#148151PurposeMammalian Expression of Hs4EBP3DepositorInsertHs4EBP3 (EIF4EBP3 Human)
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM4896 (pCV0008)
Plasmid#120081PurposeBroad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a nourseothricin resistance gene in various cyanobacteria.DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-nat1_A7120, CV-PconII-LTRBS-GFPmut2
UseOtherMutationmobA Y25FAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(deltaP)
Plasmid#83507PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
TagsHisExpressionBacterialMutationdelta . Deletion of residues 187-283PromoterT7Available SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(deltaC)
Plasmid#83506PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
TagsHisExpressionBacterialMutationdelta C. Contains residues 1-339PromoterT7Available SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6.gLacZ.Cas9-T2A-GFP
Plasmid#170361PurposeNegative control plasmid used for Crispr/cas9 based disruption.DepositorInsertCas9-T2A-GFP
UseLentiviralPromoterU6Available SinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM4889 (pCV0001)
Plasmid#120079PurposeBroad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a spectinomycin/streptomycin resistance gene in various cyanobacteria.DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
UseOtherMutationmobA Y25FAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (214-513aa)
Plasmid#78095Purposemammalian expression of GADD34 214-513 aaDepositorInsertGADD34 (PPP1R15A Human)
TagsFLAGExpressionMammalianMutationexpresses aa 214-513PromoterCMVAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRa15_436
Plasmid#49667DepositorInsertECF15_436
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only