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POC1554

Plasmid

#238471

Purpose

pMOBC360_VR: Vector Right (VR) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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H1x

Plasmid

#218111

Purpose

Encodes mus musculus histone 1 in Gateway entry vector

Depositor

Peter Hamilton

Article

Kim et al Transl Psychiatry. 2024 Jun 5;14(1):239.

Insert

Histone H1x (H1f10 Mouse)

Use

Gateway cloning entry vector

Expression

Bacterial

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–2×GFP NB(WT)

Plasmid

#232304

Purpose

KR GFP adaptor for C. elegans

Depositor

Masato Kanemaki

Article

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

KR GFP adaptor
OsTIR1(F74G)

Expression

Worm

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(WT)–GFP NB(WT)

Plasmid

#232297

Purpose

WT GFP nanbody adaptor

Depositor

Masato Kanemaki

Article

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Inserts

WT GFP adaptor
OsTIR1(F74G)

Expression

Mammalian

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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mAID(KR)–GFP NB(KR)

Plasmid

#232298

Purpose

KR GFP nanobody adaptor

Depositor

Masato Kanemaki

Article

Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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POC1525

Plasmid

#226496

Purpose

pMOBC360_VM: Vector Middle (VM) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1520

Plasmid

#221581

Purpose

pMOBK360_VR: Vector Right (VR) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1355

Plasmid

#221572

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1343, POC1344, POC1345 and POC1346; respectively

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1430

Plasmid

#221577

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1426, POC1427, POC1428 and POC1429; respectively

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1518

Plasmid

#221579

Purpose

pMOBK360_VL: Vector Left (VL) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1519

Plasmid

#221580

Purpose

pMOBK360_VM: Vector Middle (VM) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pOET3-6xHis-hSMSr-ΔSAMD

Plasmid

#236225

Purpose

Express N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cells

Depositor

Chiaki Murakami

Article

Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do

Insert

SAMD8 (SAMD8 Human)

Tags

Hexa histidine tag (6xHis-tag) and enterokinase s…

Expression

Insect

Mutation

sterile alpha motif domain at N-terminal (1-231 b…

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL678

Plasmid

#207511

Purpose

GmUbi3-SpRY-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmUBI3

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL679

Plasmid

#207512

Purpose

GmM4-SpRY-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmM4

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-AHPH

Plasmid

#187278

Purpose

Express mCherry-AHPH (RhoA biosensor)

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin and Pleckstrin homology domain (ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AHPH

Promoter

CMV

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-Cherry in pmCherryN1

Plasmid

#187291

Purpose

Express pmCherry-IL2-AH-deltaC2

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2

Promoter

CMV

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-mproSAAS (27 kDa)

Plasmid

#231635

Purpose

Mammalian expression of a flag-tagged full-length version of the mouse chaperone protein proSAAS.

Depositor

Iris Lindberg

Article

Peinado et al ACS Chem Neurosci. 2022 Jun 1;13(11):165

Insert

Pcsk1N (Pcsk1n Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGH394

Plasmid

#230851

Purpose

Ef1a-rAPOBEC1-dCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

rAPOBEC1-dCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH398

Plasmid

#230854

Purpose

Ef1a-rAPOBEC1-n'Cas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

rAPOBEC1-n'Cas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH401

Plasmid

#230852

Purpose

Ef1a-rAPOBEC1-nCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

rAPOBEC1-nCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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