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pRK5_ALFA-nb-KS

Plasmid

#238263

Purpose

For overexpression of ALFA-nb-KS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

ALFA-nb-KS

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-GFP-nb

Plasmid

#238259

Purpose

For overexpression of mCherry-GFP-nb

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-GFP-nb

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-EGFP-NPM1

Plasmid

#238268

Purpose

For overexpression of VHH05-EGFP-NPM1

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-EGFP-NPM1

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-nb-KS-FtoG

Plasmid

#238271

Purpose

For overexpression of VHH05-nb-KS-FtoG

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-nb-KS-FtoG

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-nb-noKS

Plasmid

#238269

Purpose

For overexpression of VHH05-nb-noKS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-nb-noKS

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1-KS

Plasmid

#237668

Purpose

For overexpression of mEGFP-NPM1-KS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-EWSR1-repair-tempate

Plasmid

#237682

Purpose

For knock-in msfGFP to EWSR1 locus

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EWSR1 (EWSR1 Human)

Use

CRISPR

Tags

msfGFP

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-CRTC1-MAML2-KS

Plasmid

#237672

Purpose

For overexpression of mEGFP-CRTC1-MAML2-KS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRTC1-MAML2-KS (CRTC1, MAML2 Human)

Tags

mEGFP

Expression

Mammalian

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-TAZ-CAMTA1-KS

Plasmid

#237677

Purpose

For overexpression of mEGFP-TAZ-CAMTA1-KS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TAZ-CAMTA1-KS (WWTR1, CAMTA1 Human)

Tags

mEGFP

Expression

Mammalian

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-EWS-FLI1-KS

Plasmid

#237671

Purpose

For overexpression of mEGFP-EWS-FLI1-KS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EWS-FLI1-KS (EWSR1 Human)

Tags

mEGFP

Expression

Mammalian

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-HOXA9-KS

Plasmid

#237673

Purpose

For overexpression of mEGFP-NUP98-HOXA9-KS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NUP98-HOXA9-KS (NUP98, HOXA9 Human)

Tags

mEGFP

Expression

Mammalian

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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p52K-GFP-KS

Plasmid

#237690

Purpose

For overexpression of 52K-GFP-KS

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

52K-GFP-KS

Expression

Mammalian

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b_msfGFP_stag_KS(FtoG)

Plasmid

#237631

Purpose

For expression of msfGFP_stag_KS(FtoG) in Ecoli

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

stag_KS(FtoG)

Tags

msfGFP

Expression

Bacterial

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-FUS-repair-tempate

Plasmid

#237684

Purpose

For knock-in msfGFP to FUS locus

Depositor

Denes Hnisz

Publication

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

FUS (FUS Human)

Use

CRISPR

Tags

msfGFP

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-eA3A-BE5-(NG)-PX458-EGFP

Plasmid

#229687

Purpose

CRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.

Depositor

Deborah Marsh

Publication

Dickson et al Hum Mol Genet. 2023 Jun 27:ddad105. doi:

Type

Empty backbone

Use

CRISPR

Tags

EGFP

Expression

Mammalian

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-ALFA-CD28

Plasmid

#223630

Purpose

Lentiviral expression of human CD28 with ALFA tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

ALFA tag

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(CD45TM)

Plasmid

#223629

Purpose

Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28

Plasmid

#223622

Purpose

Lentiviral expression of human CD28 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-mGFP-Eps15DN

Plasmid

#223621

Purpose

Transient expression of human Eps15 with mEGFP tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Eps15 (EPS15 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Eps15 _ 95-295aa

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SmBiT-CD80

Plasmid

#223614

Purpose

Lentiviral expression of human CD80 with SmBiT tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SmBiT

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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