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pMA3383

Plasmid

#46875

Purpose

A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3

Depositor

Mikhail Alexeyev, Troy Stevens

Publication

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1beta 3 (Gucy1b3 Rat)

Use

Retroviral

Promoter

LTR

Available since

Aug. 13, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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CCR5-donor-MCS

Plasmid

#192269

Purpose

Donor template for CRISPR Genome Editing

Depositor

Shantanu Chowdhury

Publication

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

MCS within donor template

Use

CRISPR

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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NHA-QN

Plasmid

#36441

Depositor

Edward Chan

Publication

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A (TNRC6A Human)

Expression

Mammalian

Mutation

aa 1194-1580 only

Available since

Oct. 3, 2012

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-6

Plasmid

#18024

Depositor

Jan Kitajewski

Publication

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-6 (Wnt6 Mouse)

Use

Retroviral

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-GFP-2A-SynmRuby

Plasmid

#166608

Purpose

Encodes Cre-dependent GFP-2A-synaptophysinmRuby under control of the TRE

Depositor

Martin Myers

Publication

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

GFP-2A-syn

Use

AAV

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-TVA-oG-mCherry

Plasmid

#166602

Purpose

Encodes Cre-dependent TVA-oG-mCherry under control of the TRE

Depositor

Martin Myers

Publication

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

TVA-oG-mCherry

Use

AAV

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBoxCDGC-KMet-EGFP

Plasmid

#140272

Purpose

L7Ae-dependent translational repression

Depositor

Hirohide Saito

Publication

Saito et al Nat Chem Biol. 2010 Jan;6(1):71-8. doi:

Insert

BoxCD

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBB-BT

Plasmid

#36295

Depositor

Ezra Burstein

Publication

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Tags

BT-tag (peptide sequence from P.freudenreichii tr…

Expression

Mammalian

Promoter

EF1alpha

Available since

June 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FUGW-Arl13b-PR-EGFP-TurboID

Plasmid

#248194

Purpose

Lentiviral expression of truncated Arl13b containing the Proline-Rich domain at the C-terminus (cytosol localization)

Depositor

Xuecai Ge

Publication

Liu et al Nat Commun. 2024 Apr 25;15(1):3365. doi:

Insert

The Arl13b noncilium targeting sequence-EGFP-TurboID (Arl13b Mouse)

Use

Lentiviral

Tags

EGFP and TurboID

Expression

Mammalian

Mutation

Truncated to only contain the PR domain

Promoter

hUBC

Available since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LAMP1-mCherry-FRB*

Plasmid

#207145

Purpose

Lysosome-targeted rapalog dimerizer

Depositor

Antony Galione

Publication

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

LAMP1 (LAMP1 Human)

Tags

FRB* (T2098L mutant of FKBP-rapamycin binding dom…

Expression

Mammalian

Mutation

Silent mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1355

Plasmid

#143100

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ILF3 (ILF3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble

Plasmid

#183607

Purpose

Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.

Depositor

Sally Lowell

Publication

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

LaG17 GFP nanobody SynNotch tTA

Tags

Myc and human CD8a signal sequence

Expression

Mammalian

Promoter

Mouse Pgk1

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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Stx5S-mCherry

Plasmid

#165087

Purpose

Expresses Stx5S-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Publication

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Stx5 (Short isoform) (STX5 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lacks amino acids 1-54, enocdes short isoform of …

Promoter

CMV

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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p1.2-Hyg-Fur

Plasmid

#162774

Purpose

Soluble human PACE/furin protease expression

Depositor

Ivan Vorobiev

Publication

Kovnir et al Acta Naturae. 2018 Jan-Mar;10(1):51-65.

Insert

Human PACE/furin protease soluble (FURIN Human)

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.EED

Plasmid

#125174

Purpose

Expresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Publication

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein EED (EED Human)

Tags

His-TEV

Expression

Insect

Promoter

Polyhedrin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCh-KIF17*-strep

Plasmid

#120167

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 17 (KIF17 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF17: 1-509 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28b-His-hCE

Plasmid

#112710

Purpose

Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

Depositor

Daniel Schoenberg

Publication

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNGTT (RNGTT Human)

Tags

6xHis and Stop codons placed after RNGTT sequence…

Expression

Bacterial

Mutation

R594H

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shGFP

Plasmid

#110318

Purpose

Control (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Publication

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FKBP-beta3(702-1094)-GFP

Plasmid

#100734

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Publication

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP3B1 (AP3B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

appendage and ear only

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FKBP-beta2(616-951)-mRuby2

Plasmid

#100730

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Publication

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and mRuby2

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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