We narrowed to 17,306 results for: ESC

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pHWBF01

Plasmid

#131022

Purpose

Synthetic source clone for maize Histon H2B.5 with C-terminal mCherry fusion

Depositor

Hank Bass

Article

Howe et al DNA Cell Biol. 2012 Jun;31(6):925-38. do

Insert

Histone H2B.5

Use

Cloning vector

Tags

mCherry

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTJV1Sc2-tetA

Plasmid

#166982

Purpose

Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Insert

CspRecT

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pVanCC

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-SEC61B-mEGFP

Plasmid

#115921

Purpose

donor to insert mEGFP at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-mEGFP

Use

Donor template

Tags

EGFP

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pucKan_ARS_pgkZeo_ncx1_mCherry_2TA_Puro_ARS

Plasmid

#163755

Purpose

piggybac transposon encoding human Ncx1 gene promoter-driven mCherry and puromycin-acetyltransferase for labelling and selection of pluripotent stem cell-derived cardiomyocytes

Depositor

Kurt Pfannkuche

Article

Hamad et al Methods Mol Biol. 2021 Oct 19. doi: 10.1

Insert

mCherry

Use

Piccybac transposon

Tags

puromycin-N-acetyltransferase

Expression

Mammalian

Promoter

human NCX1

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pC23-LIC-His-FLAG

Plasmid

#165477

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-FLAG-10x His

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pgaC

Plasmid

#154919

Purpose

aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pgaC

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-pnGFP-Ultra

Plasmid

#157923

Purpose

Peroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

pnGFP-Ultra, a high performance peroxynitrite sensor

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Rad18

Plasmid

#119003

Purpose

Mammalian expression of human Rad18 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Rad18

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Rad18UBD

Plasmid

#119005

Purpose

Mammalian expression of the human Rad18 Ubiquitin binding domain and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Rad18

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Cas9

Plasmid

#118998

Purpose

Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSH86

Plasmid

#127706

Purpose

Pmyo-3::GFP1-10

Depositor

David M. Miller

Article

He et al Genetics. 2019 Apr 5. pii: genetics.119.

Insert

GFP1-10

Expression

Worm

Promoter

myo-3

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSH79

Plasmid

#127704

Purpose

Pttr-39::gfp1-10

Depositor

David M. Miller

Article

He et al Genetics. 2019 Apr 5. pii: genetics.119.

Insert

GFP1-10

Expression

Worm

Promoter

ttr-39

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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p2lox-cAMPr R626A

Plasmid

#102437

Purpose

cAMPr with a point mutation R626A which theoretically decreases its affinity for cAMP

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Expression

Mammalian

Mutation

PKA-R R626A

Available Since

Feb. 23, 2019

Availability

Academic Institutions and Nonprofits only

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p9xEGFP-N1

Plasmid

#122169

Purpose

Expression of 9xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

9xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p11xEGFP-N1

Plasmid

#122171

Purpose

Expression of 11xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

11xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mCerulean3

Plasmid

#96936

Purpose

Retroviral plasmid containing mCerulean3 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Cerulean 3

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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acta 2

Plasmid

#78711

Purpose

zebrafish acta2 3-UTR–specific probe

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

acta2 (acta2 Zebrafish)

Expression

Bacterial

Mutation

contains bp 1,243–1,591

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)

Plasmid

#70198

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJB028

Plasmid

#65469

Purpose

pTrc plasmid expressing p14*-TEV

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

p14*-TEV (TEVgp1 )

Use

Synthetic Biology

Expression

Bacterial

Promoter

trc

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-rxRFP1.3

Plasmid

#67840

Purpose

redox-sensitive red fluorescent protein rxRFP1.3

Depositor

Huiwang Ai

Article

Fan et al Anal Bioanal Chem. 2016 Jan 12.

Insert

rxRFP1.3

Tags

His Tag

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only