We narrowed to 718 results for: HL;

1. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 GT>A minigene

   Plasmid

   #169274

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 1-6 GT>A minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 Exon1-6 GT>A minigene (RBM28 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NM_018077.2:c.(541+1_541+2delinsA)

   Promoter

   CMV

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF159_BRL

   Plasmid

   #225962

   Purpose

   CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-BRL (env protein)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

   Plasmid

   #119816

   Purpose

   3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

   Depositor

   Ethan Abel

   Article

   Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

   Inserts

   Luciferase
   Puromycin
   

   Use

   Lentiviral and Luciferase

   Tags

   EGFP and V5

   Expression

   Mammalian

   Mutation

   Stop codon removed in the frame of the V5 frame v…

   Promoter

   CMV/TO and Human Phosphoglycerate kinase

   Available Since

   Jan. 2, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCF142_U6-sgRNA

   Plasmid

   #225960

   Purpose

   U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   U6-sgRNA (recipient)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF153_Gag-Pol

   Plasmid

   #225961

   Purpose

   CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia Virus) Gag-Pol for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-GagPol (FMLV)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCF140_Gag-SpyCas9-NLS

   Plasmid

   #225959

   Purpose

   CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-Gag-SpyCas9-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHEE401E

   Plasmid

   #71287

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHEE401

   Plasmid

   #71286

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   sgRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

   Available Since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-26b-NbVHH05-ALFA-His

   Plasmid

   #171569

   Purpose

   Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-ALFA-His (UBE2J1 Human)
   

   Use

   Tags

   ALFA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-26b-Nb127D01-HA-His

    Plasmid

    #171566

    Purpose

    Bacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-HA-His (CXCR2 Human)
    

    Use

    Tags

    HA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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