We narrowed to 3,066 results for: AER

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pJMP8921

Plasmid

#220903

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novo

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP8932

Plasmid

#220906

Purpose

MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP580

Plasmid

#222959

Purpose

Expresses R141S K142S LoaP double mutant

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 141 to Serine and Lysine 142 to …

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP579

Plasmid

#222958

Purpose

Expresses R36S K51S LoaP double mutant

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 36 to Serine and Lysine 51 to Se…

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP578

Plasmid

#222957

Purpose

Expresses R144S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 144 to Serine

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP577

Plasmid

#222956

Purpose

Expresses K142S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Lysine 142 to Serine

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP576

Plasmid

#222955

Purpose

Expresses R141S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 141 to Serine

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP575

Plasmid

#222954

Purpose

Expresses R36S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 36 to Serine

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP573

Plasmid

#222952

Purpose

Expresses E40H LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Glutamate 40 to Histidine

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP572

Plasmid

#222951

Purpose

Expresses E40A LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Glutamate 40 to Alanine

Promoter

T7

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA4114

Plasmid

#220474

Purpose

heterologous expression of PA4114 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1472

Plasmid

#220475

Purpose

heterologous expression of PA1472 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1377

Plasmid

#220476

Purpose

heterologous expression of PA1377 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKE011

Plasmid

#207912

Purpose

Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCS018

Plasmid

#207911

Purpose

Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCS017

Plasmid

#207909

Purpose

Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS020

Plasmid

#207915

Purpose

Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKE012

Plasmid

#207914

Purpose

Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKE010

Plasmid

#207910

Purpose

Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS019

Plasmid

#207913

Purpose

Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1-2

Plasmid

#203837

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1-2

Expression

Mammalian

Mutation

KO of both AP1 sites

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b_TypeIC_acrIF2_invivo

Plasmid

#196402

Purpose

expresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

anti-CRISPR IF2 protein (orf30 P. aeruginosa)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1

Plasmid

#203835

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Expression

Mammalian

Mutation

KO of AP1 upstream

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP2

Plasmid

#203836

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Expression

Mammalian

Mutation

KO of AP1 downstream

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC6-ProC

Plasmid

#198430

Purpose

mkate2 fluorescent reporter gene under control of promoter ProC. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProC and no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRC6-ProD

Plasmid

#198431

Purpose

mkate2 fluorescent reporter gene under control of promoter ProD. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProD and no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMET7-GAG-eDHFR

Plasmid

#194635

Purpose

Mammalian expression of the eDHFR control bait in Virotrap

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Tags

ecDHFR

Expression

Mammalian

Promoter

SRalpha

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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MPC021: CamKII CMV_sNovArch_citrine (soma targeting)

Plasmid

#153194

Purpose

encoded soma targeted NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

sNovArch

Tags

citrine

Expression

Mammalian

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Promoter

tac promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS427-pCSDest2-AcrE2

Plasmid

#85677

Purpose

Mammalian expression of anti-CRISPR protein from Type I-E

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type I-E anti-CRISPR

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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