We narrowed to 12,703 results for: NUC

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pGP8GF-ZmAFB2/3 b2

Plasmid

#188425

Purpose

Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 b2

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2/3 b2

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGP8GF-ZmAFB2/3 a

Plasmid

#188423

Purpose

Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 A

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2/3 a

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGP4G-REL2N91-ccdB

Plasmid

#188444

Purpose

Zea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDEST

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

REL2N91/ccdB

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-ZmIAA8ΔPB1

Plasmid

#188432

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA8

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA8deltaPB1

Use

Cloning

Mutation

deleted PB1 domain

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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KBB441

Plasmid

#185131

Purpose

Swi6 full length with L->A mutated in nuclear localization signal

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

SWI6 deletion of amino acids 450-458

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB394

Plasmid

#185086

Purpose

NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Tags

GFP

Expression

Yeast

Mutation

Nma111 KKR 9-11 AAA; KRK 28-30 AAA

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-HypaCAS9-pA

Plasmid

#131467

Purpose

Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

Insert

Codon optimized HypaCas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJK278

Plasmid

#71134

Purpose

Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to asparagine; additional N-…

Promoter

T7

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJK320

Plasmid

#71144

Purpose

Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to serine; additional N-term…

Promoter

T7

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK319

Plasmid

#71143

Purpose

Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to alanine; additional N-ter…

Promoter

T7

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK318

Plasmid

#71142

Purpose

Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to glutamine; additional N-t…

Promoter

T7

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK315

Plasmid

#71141

Purpose

Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to glycine; additional N-ter…

Promoter

T7

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK314

Plasmid

#71140

Purpose

Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to valine; additional N-term…

Promoter

T7

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK279

Plasmid

#71135

Purpose

Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to aspartate; additional N-t…

Promoter

T7

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJK283

Plasmid

#71139

Purpose

Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine; additional N-…

Promoter

T7

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJK282

Plasmid

#71138

Purpose

Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to aspartate; additional N-t…

Promoter

T7

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJK281

Plasmid

#71137

Purpose

Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to phenylalanine; additional…

Promoter

T7

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJK280

Plasmid

#71136

Purpose

Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to phenylalanine; additional…

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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BA31_pU.CAG.SaCas9-D10A.rBGpA

Plasmid

#199251

Purpose

Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SaCas9.D10A nickase

Use

CRISPR

Tags

3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCLASP_mScarlet_CLASP_RGS2membrane

Plasmid

#133086

Purpose

Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

mScarlet-CLASP

Expression

Bacterial and Yeast

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSncg-EGFP

Plasmid

#153198

Purpose

Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93-mut

Plasmid

#87335

Purpose

To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-Cas9

Plasmid

#242759

Purpose

Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Article

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-Cas9

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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iSpyMac

Plasmid

#155014

Purpose

Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

Insert

iSpyMac

Expression

Mammalian

Mutation

PID grafted onto SpyCas9 N-terminal domain, R221K…

Promoter

EF1ɑ

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDN77

Plasmid

#61347

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS2 and NES IkBa

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(41-140)

Plasmid

#240596

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 41-140 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(36-140)

Plasmid

#240595

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 36-140 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pJK511

Plasmid

#155384

Purpose

dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Article

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR-NES

Plasmid

#175015

Purpose

Cytosol-targeted TORCAR.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

TORCAR-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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