Addgene

We narrowed to 22,177 results for: Sele

Showing: 1351 - 1380 of 22177 results

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pQTEV-PFDN2

Plasmid

#31329

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

prefoldin 2 (PFDN2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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M2371 his3::ADE2 Disruptor Converter

Plasmid

#51671

Purpose

Yeast marker swap plasmid for converting HIS3 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

ADE2

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mCitrineBb

Plasmid

#118236

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCitrine

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCitrine

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5 MEKK2 KD

Plasmid

#12183

Depositor

Gary Johnson

Article

Fanger et al EMBO J. 1997 Aug 15. 16(16):4961-72.

Insert

MEKK2 (Map3k2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Kinase dead: K385M

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pUAS-MmTFP13xmyc

Plasmid

#86371

Purpose

membrane targeted Tfp1 expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

mTfp1

Use

Zebrafish

Tags

3xMyc and GAP43 membrane targeting

Expression

Mutation

Promoter

UAS

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pME-minP:luc2

Plasmid

#138354

Purpose

Gateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBbS15c-RFP

Plasmid

#126198

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23100

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pQTEV-MTPN

Plasmid

#34829

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

myotrophin (MTPN Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shMOCS3_2

Plasmid

#102974

Purpose

Suppression of MOCS3

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shMOCS3_2 (MOCS3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shMOCS3_1

Plasmid

#102973

Purpose

Suppression of MOCS3

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shMOCS3_1 (MOCS3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBbE12k-RFP

Plasmid

#126151

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23151

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS20k-RFP

Plasmid

#126196

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

S23200

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pQTEV-STX10

Plasmid

#31572

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

syntaxin 10 (STX10 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfCFPBb

Plasmid

#118232

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfCFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfCFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-TEV-VMA Model vector

Plasmid

#86590

Purpose

This can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapI

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

VMA intein

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Original SapI site from pMal was mutated out

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pBbE15k-RFP

Plasmid

#126164

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23100

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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SI4-Jamc.2

Plasmid

#28216

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 . 8():76.

Insert

Jamc.2 mouse monoclonal antibody (jam3a Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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D588 lys2::HIS3 Disruptor Converter

Plasmid

#51675

Purpose

Yeast marker swap plasmid for converting LYS2 to HIS3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

HIS3 (HIS3 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pQTEV-SARA1

Plasmid

#34809

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

SARA1 (SAR1A Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-3×FLAG-SLC35A1

Plasmid

#186282

Purpose

transient expression of N-terminal 3×FLAG tagged SLC35A1 isoform a in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

Insert

SLC35A1 (SLC35A1 Human)

Use

Tags

3×FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0526-mCherryBb

Plasmid

#118241

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0526 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0526 promoter driven mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-Venus

Plasmid

#170518

Purpose

Plasmid containing piggyBac inverted terminal repeats (ITRs) sequences flanking CAG and Venus

Depositor

Joshua Sanes

Article

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-Venus

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_2

Plasmid

#102964

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_2 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMT440

Plasmid

#8609

Depositor

Robert Hartley

Article

Yazynin et al Gene 1996 Feb 22;169(1):131-2.

Insert

barnase, barstar

Use

Tags

as pMT416

Expression

Bacterial

Mutation

V36 codon of barnase of pMT416 replaced by polyli…

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pBSV2_PresT-mCherryBb

Plasmid

#118238

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

resT promoter driven mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaBS-sgRNA500

Plasmid

#149615

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaBS

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0026-sgRNA500

Plasmid

#149620

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

P0026

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L4-pTF-L1R

Plasmid

#45954

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Insert

pTF promoter

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

TET optimized

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-SESN2

Plasmid

#34820

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

sestrin 2 (SESN2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pQStrep2-PIR

Plasmid

#31570

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

pirin (PIR Human)

Use

Tags

His and StrepII-tag

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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