We narrowed to 4,504 results for: pcr

1. pCY 3030-02

   Plasmid

   #36215

   Depositor

   Anne Robinson

   Article

   Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

   Insert

   yEmCFP
   

   Use

   Yeast cassette for pcr and homologous recombinati…

   Tags

   yEpolylinker-yEmCFP-hphMX4

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCfB8792

   Plasmid

   #126910

   Purpose

   Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMOTag-M9-4H

   Plasmid

   #106379

   Purpose

   Can be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulation

   Depositor

   Roberto Docampo

   Article

   Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

   Insert

   GlmS with M9 mutation
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pgRNA-guideless

   Plasmid

   #129464

   Purpose

   Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   Guideless gRNA backbone
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 (SpeI)

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCY 3080-01

   Plasmid

   #36226

   Depositor

   Anne Robinson

   Article

   Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

   Insert

   yEmCitrine
   

   Use

   Yeast cassette for pcr and homologous recombinati…

   Tags

   yEpolylinker-yEmCitrine-kanMX

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPOTv7-blast-mScarletI

   Plasmid

   #201442

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   mScarlet-I
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPOTv7-blast-mCardinal

   Plasmid

   #179796

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Keith Gull, Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   mCardinal
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSNAPf-AP

   Plasmid

   #61830

   Purpose

   Encodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPull

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

   Insert

   SNAP-AP
   

   Use

   Pcr template

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLO104

   Plasmid

   #90224

   Purpose

   Complements mutations in Aspergillus nidulans gene riboB (AN0670) (mutants require riboflavin)

   Depositor

   Berl Oakley

   Article

   Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

   Insert

   ATET_00531
   

   Use

   Aspergillus

   Tags

   Expression

   Mutation

   Promoter

   pLac

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. XX>sgRNA F+E

    Plasmid

    #87543

    Purpose

    Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

    Depositor

    Lionel Christiaen

    Article

    Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCY 3110-01

    Plasmid

    #36236

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    mEos2
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-mEos2-kanMX

    Expression

    Mutation

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pPOTv7-hygro-BFP

    Plasmid

    #190260

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Sue Vaughan

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    BFP
    

    Use

    For endogenous gene tagging

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPOTv7-g418-mScarlet

    Plasmid

    #179823

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mScarlet
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPOTv7-blast-nanoluc

    Plasmid

    #179797

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    nanoluc
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPOTv7-g418-eYFP

    Plasmid

    #190220

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    eYFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPOTv7-hygro-mCherry

    Plasmid

    #190262

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Sue Vaughan

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mCherry
    

    Use

    For endogenous gene tagging

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L0-I39 PhiC31-Intein GG1-GG4

    Plasmid

    #195905

    Purpose

    Level 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    PhiC31-intein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L0-I40 Intein-PhiC31 GG1-GG4

    Plasmid

    #195906

    Purpose

    Level 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    Intein-PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCY 3040-01

    Plasmid

    #36217

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    yEGFP
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-yEGFP-kanMX

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPOTv7-blast-BFP

    Plasmid

    #190258

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Sue Vaughan

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    BFP
    

    Use

    For endogenous gene tagging

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPOTv6.1-phleo-3Myc:mNG:3Myc

    Plasmid

    #181937

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    mNG
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPOTv7-blast-10Ty

    Plasmid

    #179807

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    10xTy
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPOTv7-hygro-10Ty

    Plasmid

    #179808

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    10xTy
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGJJ055

    Plasmid

    #183622

    Purpose

    ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCY 3010-05

    Plasmid

    #36211

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    LEU2
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-Cerulean-LEU2

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLO101

    Plasmid

    #90221

    Purpose

    Complements mutations in Aspergillus nidulans gene biA (AN6644) (mutants require biotin)

    Depositor

    Berl Oakley

    Article

    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

    Insert

    ATET_07102
    

    Use

    Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    pLac

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLO102

    Plasmid

    #90222

    Purpose

    Complements mutations in Aspergillus nidulans gene choA (AN2154) (mutants require choline)

    Depositor

    Berl Oakley

    Article

    Dohn JW et al Fungal Genet Biol. 2018 Feb;111:1-6. doi

    Insert

    ATET_02347
    

    Use

    Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    pLac

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBALU1

    Plasmid

    #69316

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfp

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    GFPint-FgF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCY 3090-02

    Plasmid

    #36231

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    yEpolylinker
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-mCherry-hphMX4

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBALU6

    Plasmid

    #69321

    Purpose

    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: Venus in frame w/ FRT-galK-FRT; no introns; N-terminal fusion

    Depositor

    Oliver Hobert

    Article

    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

    Insert

    Venus-FgF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only