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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,272 results for: aaas

Showing: 1801 - 1840 of 3272 results

  1. pJK304

    Plasmid
    #72225
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala368>Asp mutant (H6AaAlr-A368D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes alanine-368 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72225-plasmid-map-sequence-id-134624

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  2. pJK303

    Plasmid
    #72224
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala367>Asp mutant (H6AaAlr-A367D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes alanine-367 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72224-plasmid-map-sequence-id-134623

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  5. RepX_pET28a(+)

    Plasmid
    #172199
    Purpose
    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.
    Depositor
    Taekjip Ha
    Article
    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do
    Insert
    RepX superhelicase
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    C18L, C43S, C167V, S400C, C612A
    Promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    172199-plasmid-map-sequence-id-356977

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  7. pSUC01

    Plasmid
    #177204
    Purpose
    chromogenic reporter for gene knockout in Streptomyces
    Depositor
    Wenjun Guan
    Article
    Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1
    Insert
    SaindC
    Use
    Synthetic Biology
    Tags
    NONE
    Expression
    Bacterial
    Mutation
    NONE
    Promoter
    TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    177204-plasmid-map-sequence-id-382362

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  8. pLKO-Tet-On-ARNTL-shRNA2

    Plasmid
    #166914
    Purpose
    Lentivirus for inducible knockdown of human ARNTL(BMAL1)
    Depositor
    Roland Friedel
    Insert
    ARNTL shRNA (BMAL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166914-plasmid-map-sequence-id-331230

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  10. pLKO-FOXM1-shRNA-84

    Plasmid
    #89494
    Purpose
    Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.
    Depositor
    Adam Karpf
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    89494-plasmid-map-sequence-id-180323

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  17. 6XKC

    Plasmid
    #162271
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    FEM1C (FEM1C Human)
    Use
    Tags
    6xHis, GST and Thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    GST-F:tgaagctatcccacaaattg
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162271-plasmid-map-sequence-id-319836

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  19. pJK350

    Plasmid
    #71560
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala365>Asp mutant (H6AaAlr-A365D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    mutation changes alanine-365 to aspartate
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71560-plasmid-map-sequence-id-134627

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  20. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid
    #214688
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Puromycin resistance cassette has silent mutation…
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214688-plasmid-map-sequence-id-428817

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  21. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118159
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118159-plasmid-map-sequence-id-240446

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  22. pJK290

    Plasmid
    #71554
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with double mutant (AaAlr-K39A,Y260A)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes lysine-39 to alanine and tyrosine-260 to …
    Promoter
    T7
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    71554-plasmid-map-sequence-id-139946

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  23. pJK326

    Plasmid
    #71557
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-368 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71557-plasmid-map-sequence-id-134622

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  24. pJK325

    Plasmid
    #71556
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes alanine-367 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71556-plasmid-map-sequence-id-131415

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  25. pJK345

    Plasmid
    #71559
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 and alanine-368
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71559-plasmid-map-sequence-id-134626

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  26. pJK344

    Plasmid
    #71558
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    deletes alanine-367 (same as deleting alanine-368)
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    71558-plasmid-map-sequence-id-134625

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  27. p201N 5770

    Plasmid
    #55772
    Purpose
    Contains soybean miRNA miR5770 recognition sequence (TCTTGTCCAAACCATAGTCCAA) to produce siRNAs from 3' target sequences and induce RNA silencing
    Depositor
    Wayne Parrott
    Article
    Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    GmUbi
    Available since
    Sept. 26, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55772-plasmid-map-sequence-id-86514

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  29. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid
    #82327
    Purpose
    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)
    Depositor
    Elizabeth Stoll
    Insert
    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82327-plasmid-map-sequence-id-155713

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  30. pAAV-CMV-FLEX-SaCas9-U6-sgDrd1

    Plasmid
    #159917
    Purpose
    Mutagenesis of Drd1
    Depositor
    Larry Zweifel
    Insert
    Drd1 (Drd1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159917-plasmid-map-sequence-id-317329

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  31. GFP_CD47_LU

    Plasmid
    #65474
    Purpose
    GFP tagged CD47 with its own long 3'UTR
    Depositor
    Christine Mayr
    Article
    Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature
    Inserts
    Signal peptide of CD47
    GFP
    CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    65474-plasmid-map-sequence-id-117297

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  36. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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Showing: 1801 - 1840 of 3272 results