We narrowed to 156,890 results for: Des

1. pDEST-HemmarG

   Plasmid

   #31221

   Depositor

   Yuh-Nung Jan

   Article

   Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

   Insert

   CD4-tdGFP (CD4 Human, Fly, Aequorea victoria)
   

   Use

   Tags

   CD4 and EGFP/GFP

   Expression

   Insect

   Mutation

   Fusion of signal peptide of Drosophila Akh gene, …

   Promoter

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFRT-DestFLAGHA_HuD

   Plasmid

   #65760

   Purpose

   constitutively expresses FLAG-HA-tagged HuD in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

3. 1455_pDEST_miniTol2_R4-R2_MCS

   Plasmid

   #171794

   Purpose

   pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST14-SpyCatcher EQ

   Plasmid

   #35045

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyCatcher EQ
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Deletion of C-terminal till I113, and point mutat…

   Promoter

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pDEST47-SAR1

   Plasmid

   #67472

   Purpose

   Mammalian expression of hSar1 with stop codon (native expression)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SAR1 (SAR1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA-pDEST_TMEM192_BirA_Flag

   Plasmid

   #227958

   Purpose

   BioID experiment

   Depositor

   Alessandro Ori

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Tags

   BirA-FLAG

   Expression

   Mammalian

   Mutation

   R118G in BirA

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDest-557

   Plasmid

   #161890

   Purpose

   Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions in E. coli

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDest-636

   Plasmid

   #159574

   Purpose

   Gateway Destination vector for protein expression in baculovirus

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Type

   Empty backbone

   Use

   Tags

   His6-MBP

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST40-MFRN2

   Plasmid

   #50546

   Purpose

   Expression of MFRN2 gene in mammalian cells

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   Mrfn2 (SLC25A28 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pDEST14-SpyCatcher002 EQ

    Plasmid

    #102830

    Purpose

    Bacterial expression of unreactive version of SpyCatcher002

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyCatcher002 EQ
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    E77Q mutation prevents isopeptide bond formation

    Promoter

    T7

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFRT-DestFLAGHA_RNAseH1

    Plasmid

    #65783

    Purpose

    constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    RNaseH1 (RNASEH1 Human, Zebrafish)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST17-UbE2U

    Plasmid

    #15810

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2U (UBE2U Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pDEST-ERBB3-v1

    Plasmid

    #154893

    Purpose

    Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Tags

    Venus fragment 1 (v1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST_6xUAS:Lamp1-mGFP

    Plasmid

    #192356

    Purpose

    Lamp1 tagged with mGFP, expressed under the UAS promoter

    Depositor

    Francesca Peri

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    Lamp1 (LAMP1 Human)
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLEX_970_puro_DEST_V5_TLOC1 (399aa)

    Plasmid

    #45288

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pFRT-DestFLAGHA_HuC

    Plasmid

    #65759

    Purpose

    constitutively expresses FLAG-HA-tagged HuC in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL3 (ELAVL3 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMMDS DEST

    Plasmid

    #206264

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Cre-donor, multimate/gateway dest vector (multiba…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Munc18cwt-pDEST27

    Plasmid

    #85626

    Purpose

    GST tagged Munc18c

    Depositor

    David James

    Article

    Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

    Insert

    Munc18c (Stxbp3 Mouse)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMB-PDESr

    Plasmid

    #61207

    Purpose

    Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtPDLP1
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST47-ARL8B

    Plasmid

    #67467

    Purpose

    Mammalian expression of hArl8b with stop codon (native expression)

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only