We narrowed to 4,390 results for: NAP

1. pFsynW SYB2 reporter

   Plasmid

   #197276

   Purpose

   Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYB2 and HaloTag (Vamp2 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   N/A

   Promoter

   hSyn (human synapsin I promoter)

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-CD4-FLAG

   Plasmid

   #51604

   Purpose

   expresses CD4 with C-terminal FLAG tag

   Depositor

   Suzanne Paradis

   Article

   Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

   Insert

   CD4 (CD4 Human)
   

   Use

   Tags

   FLAG and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pF(UG) hSyn SYT7alpha-HaloTag

   Plasmid

   #179715

   Purpose

   Lentiviral expression of mouse Syt7 with C terminal HaloTag

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-tdTomato-P2A-paCaMKII(K42M)

   Plasmid

   #165433

   Purpose

   Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   tdTomato-P2A-paCaMKII(K42M)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   K42M: Kinase dead mutation

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI syn iGluSnFR

   Plasmid

   #106123

   Purpose

   Indicator for glutamate imaging (synapsin promoter)

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR
   

   Use

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI pHluorin Syt11

   Plasmid

   #184512

   Purpose

   Mammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 11 (Syt11 Mouse)
   

   Use

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. VAMP2-12aa-mCit

   Plasmid

   #53237

   Purpose

   Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Tags

   12aa-linker and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pF(UG) hSyn PP-HaloTag-SYT7alpha

   Plasmid

   #179714

   Purpose

   Lentiviral expression of mouse Syt7 with N terminal HaloTag

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF(UG) hSyn PM-SYT7alpha deltaTMD

   Plasmid

   #179720

   Purpose

   Lentiviral expression of plasma membrane-targeted mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF NS34a-msGFP-'S-mito

    Plasmid

    #158765

    Purpose

    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. GalT-moxDendra2

    Plasmid

    #89789

    Purpose

    mammalian expression of GC localized moxDendra2

    Depositor

    Erik Snapp

    Article

    Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

    Insert

    GalT signal anchor transmembrane domain (B4GALT1 Human)
    

    Use

    Tags

    moxDendra2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p045

    Plasmid

    #70057

    Purpose

    Co-expresses Syt1-SNARE37aa-linker complex with pDC184

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

    Inserts

    C2AB-linker-SNAP-25_C
    SNAP-25_N
    

    Use

    Tags

    No tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG

    Plasmid

    #167477

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG

    Plasmid

    #167476

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(T286A)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CVS-N2c-SypRFP

    Plasmid

    #172381

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    Synaptophysin1-RFP fusion protein
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-myc-CD4-Sema4D

    Plasmid

    #51603

    Purpose

    expresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    CD4-Sema4D
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Sema4D amino acids 1-754 have been replaced with …

    Promoter

    CMV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pWPXL-resNP1

    Plasmid

    #65061

    Purpose

    Constitutive expression of NP1 resistant to silencing by RNAi with pLVTH-shNP1

    Depositor

    Ramon Trullas

    Article

    Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

    Insert

    Neuronal Pentraxin 1 (Nptx1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G447A, C450T, A451T, G452C, C454A, C456A, C459G

    Promoter

    EF1alpha

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRIG-myc-Sema4DdeltaC

    Plasmid

    #51607

    Purpose

    expresses myc-tagged Sema4D lacking C terminus in lentiviral backbone

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    Sema4DdeltaC (Sema4d Mouse)
    

    Use

    Lentiviral

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    deleted last 70 amino acids in the C-terminus

    Promoter

    RSV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

    Plasmid

    #179717

    Purpose

    Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET SUMO-syt1 (96-421)

    Plasmid

    #213619

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 (96-421)

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 (96-421) (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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