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We narrowed to 1,293 results for: Ncl

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Showing: 901 - 920 of 1293 results

  1. pTS2354_Tier3(PB)-Ruby_PuroR

    Plasmid
    #169653
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mRuby2 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169653-plasmid-map-sequence-id-366195

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  2. Ash1

    Plasmid
    #169711
    Purpose
    Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)
    Depositor
    Tanja Mittag
    Article
    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323
    Insert
    Ash1 (ASH1 Budding Yeast)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation including encoding amino acids 420-500
    Promoter
    T7
    Available Since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169711-plasmid-map-sequence-id-334782

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  6. pAL832

    Plasmid
    #119305
    Purpose
    precusor outer membrane protein A, ompA, truncated after amino acid 176
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
    Tags
    none
    Expression
    Bacterial
    Mutation
    truncated after amino acid 176, mutations Threoni…
    Promoter
    lac
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119305-plasmid-map-sequence-id-232460

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  8. miR-451 Trap

    Plasmid
    #61487
    Purpose
    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    miR-451 Trap
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    bidirectional phosphoglycerate kinase promoter (b…
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pHL 990

    Plasmid
    #52992
    Purpose
    pLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    RhyB sRNA (competitor)
    rpoS
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    includes −150 to +30 bp relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pET30-GBF-E6N

    Plasmid
    #21699
    Depositor
    James Baleja
    Article
    Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.
    Insert
    Protein G domain B1 fused N-terminal zinc binding domain of human papillomavirus type 16 (residues 2-74) (E6 Human papillomavirus type 16)
    Tags
    Protein G B1 domain
    Expression
    Bacterial
    Mutation
    GB1 (1-56aa) fusion tag with mutations at three p…
    Available Since
    July 28, 2009
    Availability
    Academic Institutions and Nonprofits only

  14. pLVX-TetOne-puro-atpG

    Plasmid
    #248067
    Purpose
    Lentiviral expression of gamma subunit of ATPGobble. Includes ampR and puroR (puromycin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-bleo-atpD (#248066) for active ATPGobble.
    Depositor
    Denis Titov
    Article
    Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.
    Insert
    atpG (atpG E. coli)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    codon-optimized for expression in human cells
    Promoter
    TRE3GS
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    248067-plasmid-map-sequence-id-493799

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  19. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and tcp830
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

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  20. pLGB36

    Plasmid
    #135621
    Purpose
    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection
    Depositor
    Laurie Comstock
    Article
    Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-
    Insert
    bfe3
    Use
    Bacteroides suicide vector with inducible counter…
    Tags
    signal sequence for periplasmic targeting
    Promoter
    Bacteroides promoter regualted by TetR transcript…
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    135621-plasmid-map-sequence-id-267063

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Showing: 901 - 920 of 1293 results