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Plasmid#140199PurposeEpisomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.DepositorInsertdSpCas9-VPR
UseCRISPR and Synthetic Biology; Expression in asper…TagsVPR (VP64-p65-Rta), NLSExpressionMutationPromoterPgpdAAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC
Plasmid#140197PurposeEpisomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.DepositorInsertdLbCas12a-VPR
UseCRISPR and Synthetic Biology; Expression in asper…TagsVPR (VP64-p65-Rta), NLSExpressionMutationD832A DNase deactivatedPromoterPgpdAAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSP2440
Plasmid#72250PurposeHuman expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…PromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GRET
Plasmid#158222PurposeControl lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.DepositorInsertGFP-Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-gfaABC1D-monSTIM1
Plasmid#161797PurposeLentiviral vector expressing monSTIM1 in astrocytes.DepositorInsertEGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromotergfaABC1DAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAX APPs-751-alpha
Plasmid#30149DepositorInsertAmyloid Precursor Protein - soluble (APP Human)
UseTagsExpressionMammalianMutationsplice variant 751; amino acids 1–612PromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)
Plasmid#114068PurposeT7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guidesDepositorInsertentry vector for LbCas12a crRNAs
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer
Plasmid#66779PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertshbeRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CamKIIa-monSTIM1
Plasmid#161796PurposeLentiviral vector expressing monSTIM1 in neurons.DepositorInsertEGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterCamKIIaAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MLM3720
Plasmid#49944PurposeExpresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a controlDepositorInsert3x FLAG Tet1CDmut RH-3 (RHOXF2 Human)
UseTALENTags3x FLAGExpressionMutationcatalytic domain of TET1 inactive (H1671Y and D16…PromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/FKBP12-pBirA(284-C)-HA
Plasmid#154452Purposeexpresses FKBP12-pBirA(284-C)-HA/biochemical assayDepositorInsertFKBP12-pBirA(284-C)-HA (FKBP12 Human)
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgTsc1#1/Cre
Plasmid#173657PurposeExpresses a Tsc1-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Tsc1 (Tsc1 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgTsc1#2/Cre
Plasmid#173658PurposeExpresses a Tsc1-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Tsc1 (Tsc1 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgPtprd#2/Cre
Plasmid#173648PurposeExpresses a Ptprd-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Ptprd (Ptprd Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgPtprd#1/Cre
Plasmid#173647PurposeExpresses a Ptprd-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Ptprd (Ptprd Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MLM3709
Plasmid#49943PurposeExpresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsert3x FLAG Tet1CD RH-3 (RHOXF2 Human)
UseTALENTags3x FLAGExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
JA740
Plasmid#49939PurposeExpresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in EGFP for use as a controlDepositorInsertTet1CD GFP (TET1 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
MLM3713
Plasmid#49946PurposeExpresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsert3x FLAG Tet1CD RH-4 (RHOXF2 Human)
UseTALENTags3x FLAGExpressionMutationPromoterEF1aAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/FKBP12-pBirA(79-C)-ha
Plasmid#154449Purposeexpresses FKBP12-pBirA(79-C)-ha/biochemical assayDepositorInsertFKBP12-pBirA(79-C)-ha (FKBP12 Human)
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
MLM3762
Plasmid#49947PurposeExpresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a controlDepositorInsert3x FLAG Tet1CDmut RH-4 (RHOXF2 Human)
UseTALENTags3x FLAGExpressionMutationcatalytic domain of TET1 inactive (H1671Y and D16…PromoterEF1aAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only