We narrowed to 1,270 results for: grna cloning vector

1. Circular 200,100 (mIDUA)

   Plasmid

   #170123

   Purpose

   AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (Idua Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6 and mouse U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Circular 200,100 (GAPDH)

   Plasmid

   #170120

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (GAPDH Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMonAID_nCas9-PmCDA_Hyg_ALS

   Plasmid

   #91693

   Purpose

   Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAX198

   Plasmid

   #173042

   Purpose

   Custom vector for sgRNA library construction

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDicAID_nCas9-PmCDA-2A-NptII_ETR

   Plasmid

   #91695

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pY036_ATP1A1_G5_Array

   Plasmid

   #86620

   Purpose

   Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMonAID_dCas9-PmCDA_Hyg_ALS

   Plasmid

   #91692

   Purpose

   Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A and H840A for dead Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY036_ATP1A1_G3_Array

   Plasmid

   #86619

   Purpose

   Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
   

   Use

   CRISPR; Co-selection via nhej using ouabain

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

   Plasmid

   #214877

   Purpose

   Lentiviral vector encoding RfxCas13d targeting LPT guide array

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crLPT-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-H1-sgGFP1-7SK-sgCas9

    Plasmid

    #87908

    Purpose

    multiple sgRNA

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgCas9
    

    Use

    Gateway entry vector

    Promoter

    H1, 7SK

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. SAM-DNMT3A+sgGFP

    Plasmid

    #213165

    Purpose

    Vector with sgGFP for induction of global DNA methylation.

    Depositor

    Sefi Rosenbluh

    Article

    Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

    Insert

    sgGFP
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    E1Fa and U6 and PGK

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SAM-DNMT3A+sgAAVS1_145

    Plasmid

    #213166

    Purpose

    Vector with sgAAVS1 for induction of global DNA methylation.

    Depositor

    Sefi Rosenbluh

    Article

    Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

    Insert

    sgAAVS1_145
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    E1Fa and U6

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SAM-DNMT3A+sgAAVS1_118

    Plasmid

    #213167

    Purpose

    Vector with sgAAVS1 for induction of global DNA methylation.

    Depositor

    Sefi Rosenbluh

    Article

    Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

    Insert

    sgAAVS1_118
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    E1Fa and U6

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SAM-DNMT3A-inactive+sgGFP

    Plasmid

    #213169

    Purpose

    Vector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

    Depositor

    Sefi Rosenbluh

    Article

    Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

    Insert

    sgGFP
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    E1Fa and U6

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)

    Plasmid

    #214882

    Purpose

    Lentiviral vector encoding RfxCas13d targeting PROX guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crPROX-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SA-linker-P2A-lifeact-mNeongreen-ORF-1

    Plasmid

    #241204

    Purpose

    Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

    Depositor

    Junsu Kang

    Insert

    lifeact-mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SA-linker-P2A-lifeact-mNeongreen-ORF-2

    Plasmid

    #241205

    Purpose

    Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

    Depositor

    Junsu Kang

    Insert

    lifeact-mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SA-linker-P2A-lifeact-mNeongreen-ORF-3

    Plasmid

    #241206

    Purpose

    Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

    Depositor

    Junsu Kang

    Insert

    lifeact-mNeongreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF3

    Plasmid

    #241182

    Purpose

    Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

    Depositor

    Junsu Kang

    Insert

    NTRv2; mNeonGreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF1

    Plasmid

    #241180

    Purpose

    Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

    Depositor

    Junsu Kang

    Insert

    NTRv2; mNeonGreen
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge