We narrowed to 6,573 results for: tac

1. MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5

   Plasmid

   #135455

   Purpose

   Bacterial expression of uTEV1Δ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
   

   Use

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability, S153N mutation…

   Promoter

   tac promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-6P-1 Stu2 1-590

   Plasmid

   #38314

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-4T1-RhoA G17A

   Plasmid

   #69357

   Purpose

   expression of nucleotide free RhoA in bacterial cells

   Depositor

   Rafael Garcia-Mata

   Article

   Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G17A

   Promoter

   tac

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pGEX6P1-hPREP

   Plasmid

   #179387

   Purpose

   Express human prolyl oligopeptidase (tagged) in bacterial cells

   Depositor

   Christian Gruber

   Article

   Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

   Insert

   human prolyl oligopeptidase (PREP Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   TAC

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO.1-scrCdh20.1 GFP

   Plasmid

   #209106

   Purpose

   GFP expressing scramble of shRNA targeting Cdh20

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   scrCdh20.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

   Plasmid

   #135454

   Purpose

   Bacterial expression of TEVΔ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
   

   Use

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability. Truncated form…

   Promoter

   tac promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-6P-1 Stu2 1-306

   Plasmid

   #38315

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 918 bp from STU2 ORF (STU2 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-HA-GlpG

   Plasmid

   #155135

   Purpose

   Expresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-KG-CAND1

   Plasmid

   #19949

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

    Plasmid

    #222989

    Purpose

    Bacterial expression of iGeoCas9(G) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(G)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX2TK-Osh2-PH

    Plasmid

    #71370

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh2-PH
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 277-398

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pGEX2TK-Num1-PH

    Plasmid

    #71369

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Num1-PH
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa2563-2692

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pGEX2TK-Skm1-PH

    Plasmid

    #71371

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Skm1-PH
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa1-132

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-4T1-Cdc42 G15A

    Plasmid

    #69356

    Purpose

    expression of nucleotide free Cdc42 in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G15A

    Promoter

    tac

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pSub-MBP-GFP(2M-IKQE)

    Plasmid

    #185757

    Purpose

    Bacterial expression of MBP-GFP(2M IKQE)

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    GFP(2M-IKQE)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GST-N1IC

    Plasmid

    #47612

    Purpose

    bacterial expression of GST-myc-Notch1 IC

    Depositor

    Urban Lendahl

    Article

    Beatus et al Development. 1999 Sep;126(17):3925-35.

    Insert

    Notch1 intracellular domain (Notch1 Mouse)
    

    Use

    Tags

    GST and myc

    Expression

    Bacterial

    Mutation

    contains amino acids 1753-2531

    Promoter

    tac

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

17. T7 promoter + rpsL reporter plasmid

    Plasmid

    #156455

    Purpose

    A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    rpsL
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    tac and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENV8(GAAA)-GFPuv

    Plasmid

    #70128

    Purpose

    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop

    Depositor

    Robert Batey

    Article

    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

    Insert

    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PGEX 4T-1-PPARgamma2

    Plasmid

    #78773

    Purpose

    To purify GST-tagged recombinant protein

    Depositor

    Jaewhan Song

    Article

    Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

    Insert

    PPAR_2
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

20. GST eIF2beta

    Plasmid

    #45900

    Depositor

    Louise Larose

    Article

    Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

    Insert

    eIF2S2 (Eif2s2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only