We narrowed to 18,937 results for: Tec

1. SIK2 gRNA (BRDN0001149208)

   Plasmid

   #77138

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SIK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SIK2 (SIK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SIK1 gRNA (BRDN0001145248)

   Plasmid

   #77895

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SIK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SIK1 (SIK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCHKO_PDPR_exon_deletion_1

   Plasmid

   #155065

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Venus-cpVenus-FLARE-CKAR

   Plasmid

   #123343

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-CKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SIK3 gRNA (BRDN0001144776)

   Plasmid

   #75754

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SIK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIK3 gRNA (BRDN0001148297)

   Plasmid

   #75755

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SIK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SIK3 gRNA (BRDN0001148836)

   Plasmid

   #75756

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SIK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SIK3 gRNA (BRDN0001149197)

   Plasmid

   #75757

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SIK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

   Plasmid

   #133793

   Purpose

   U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   CjeCas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   V2-TGAA linker sgRNA architecture from Kim et al.…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-hA1-BE3

    Plasmid

    #113418

    Purpose

    Expresses hA1-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA1-BE3 (APOBEC1 S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307-IBV-3CL-C143A

    Plasmid

    #160289

    Purpose

    Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C143A

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX028

    Plasmid

    #167151

    Purpose

    MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnHispS-ocsT
    

    Expression

    Plant

    Promoter

    p35s

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRIB2 gRNA (BRDN0001148332)

    Plasmid

    #75604

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIB2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIB2 (TRIB2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET-A2-77p-Venus

    Plasmid

    #64856

    Purpose

    Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

    Depositor

    Sriram Neelamegham

    Article

    Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

    Insert

    VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Synthetic, Human)
    

    Tags

    7X His and Venus

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. RFK gRNA (BRDN0001146181)

    Plasmid

    #76847

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RFK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RFK (RFK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. RFK gRNA (BRDN0001162491)

    Plasmid

    #76850

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RFK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RFK (RFK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgRNA BRCA1 Q1779Stop

    Plasmid

    #139331

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 Q1779Stop using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLR5-CAG-NLS-tdiRFP670-2A-LBR

    Plasmid

    #82620

    Purpose

    Expresses NLS-tdiRFP-2A-LBR in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    NLS-tdiRFP670-LBR (Lbr Mouse, Synthetic)
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-BE3-P2A-GFP-PGK-Puro

    Plasmid

    #110867

    Purpose

    Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-VQRRA-P2A-Puro

    Plasmid

    #110851

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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