We narrowed to 9,399 results for: pho;

1. pMXS-IRES-BLAST flag-CAD M1601E

   Plasmid

   #169828

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   M1601E; TCCC -> AGTC silent mutations at nt527…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. IL2-mCherry-AH in pmCherryN1

   Plasmid

   #187284

   Purpose

   Express IL2-AH-mCherry

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   AH

   Promoter

   CMV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Venus-Bad-2A-pEGFP-C1

   Plasmid

   #166748

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domain

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   Bad-2A (BAD Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   2 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Venus-Puma-4E-pEGFP-C1

   Plasmid

   #166746

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Puma-4E (BBC3 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   4 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Venus-tBid-2E-pEGFP-C1

   Plasmid

   #166747

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation to 2 positions in the BH3 domain

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   tBid-2E (Bid Mouse)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   2 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLuc-Optop38i-dsm

   Plasmid

   #89748

   Purpose

   Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   Optop38i dark-state mutant
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   C450A mutation of AsLov2Jalpha

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEAHISPA4631

   Plasmid

   #97017

   Purpose

   Expressing Pseudomonas aeruginosa orf PA4631 protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Nucleoside-diphosphate-sugar epimerase
   

   Tags

   N-ter TEV protease cleavable 6HIs

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CSKP_HUMAN_D0

   Plasmid

   #79714

   Purpose

   This plasmid encodes the kinase domain of CSKP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSKP (CASK Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pMSCV40-EF1a-hBCL10(1-114)-SO

   Plasmid

   #75257

   Purpose

   retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   Bcl10 (BCL10 Human)
   

   Use

   Retroviral

   Tags

   StrepOne Tag

   Expression

   Mammalian

   Mutation

   AS 1-116 (CARD-domain)

   Promoter

   pMSCV-LTRs, EF1a

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX307/PTPN11

    Plasmid

    #140941

    Purpose

    expresses PTPN11

    Depositor

    Kimberly Stegmaier

    Article

    Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

    Insert

    PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223-NME6

    Plasmid

    #23562

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    NME6 (NME6 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pET30-2-GPD1

    Plasmid

    #83911

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLPC-dnMAML1-mVenus

    Plasmid

    #171023

    Purpose

    Retroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    MAML1 Dominant Negative (MAML1 Human)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    amino acids 12-74 only, hence conferring dominant…

    Promoter

    CMV

    Available Since

    April 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid

    #236007

    Purpose

    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    D835Y substitution

    Promoter

    CMV

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFORF1355

    Plasmid

    #143100

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ILF3 (ILF3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET30-2-GPD1L

    Plasmid

    #83912

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

    Plasmid

    #135814

    Purpose

    Fluorescent reporter for knock-in of TdTomato into the TH locus

    Depositor

    Tim Ahfeldt, Lee Rubin

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
    

    Tags

    p2a-TdTomato

    Expression

    Mammalian

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TFORF0117

    Plasmid

    #141524

    Purpose

    Lentiviral vector for overexpressing the FOXM1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF0118

    Plasmid

    #141525

    Purpose

    Lentiviral vector for overexpressing the FOXM1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid

    #162876

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    GFP Binding Protein tagged with mEos2
    

    Tags

    mEos2

    Expression

    Mammalian

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge