159,617 results

1. pFB2D

   Plasmid

   #195286

   Purpose

   Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   DTR EGFP 2A Puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDB179-TurboPETase

   Plasmid

   #218693

   Purpose

   Encodes codon optimized TurboPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   TurboPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmirGLO-3'UTR-TNF

   Plasmid

   #153069

   Purpose

   Human TNF 3'UTR region cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   3' UTR region of TNF gene (TNF Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti SYN-SVI-DIO-dCas9-VPR

   Plasmid

   #164576

   Purpose

   Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

   Depositor

   Jeremy Day

   Article

   Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

   Insert

   A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT22-GluCNZ-6His

   Plasmid

   #213504

   Purpose

   Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

   Depositor

   John Denu

   Article

   Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

   Insert

   Glucanase (glucan endo-1,3-beta-glucosidase)
   

   Use

   Tags

   6His tag

   Expression

   Bacterial

   Mutation

   1-36 Signal Sequence Deletion

   Promoter

   T7

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PGK-EGFP-FKBPF36V

   Plasmid

   #199765

   Purpose

   plasmid for targeting dTAG into the AAVS1 locus

   Depositor

   Hannes Lans

   Article

   van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

   Insert

   GFP-dTAG
   

   Use

   Tags

   GFP and dTAG

   Expression

   Mammalian and Worm

   Mutation

   Promoter

   hPGK

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDY1314_RADARS_Luciferase

   Plasmid

   #193191

   Purpose

   Express Gaussia Luciferase conditional upon RNA transcript

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   RADARS Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

   Plasmid

   #221899

   Purpose

   Expresses alpha tubulin fused to mStayGold

   Depositor

   Janelia Integrative Imaging

   Insert

   mStayGold-aTubulin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH2B_mCherry_IRES_puro2

   Plasmid

   #21045

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   pH2B (H2BC16P Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

    Plasmid

    #84469

    Purpose

    pAAV-RAM-EGFP

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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