162,408 results

1. pcDNA3-SARS-CoV-2-S-RBD-8his

   Plasmid

   #145145

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
   

   Use

   Tags

   8his tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-Cas9-gRNA-TagBFP2

   Plasmid

   #124774

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSET-6xTR-TUBE

   Plasmid

   #110313

   Purpose

   E. coli expression and purification of 6xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   6xTR-TUBE (UBQLN1 Human)
   

   Use

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a-dCas9-KRAB-GFP backbone

   Plasmid

   #194281

   Purpose

   Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

   Plasmid

   #179686

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T1413I

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-nCas9n-nanos 3’UTR

   Plasmid

   #62542

   Purpose

   expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

   Insert

   nanos 3' UTR (nanos3 Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   3' UTR only

   Promoter

   SP6

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. APEX2-V5-G3BP1_pTRE-G418

   Plasmid

   #202013

   Purpose

   expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   APEX2-V5-G3BP1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-Tight

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-NLS-AcrIIA4

   Plasmid

   #113037

   Purpose

   Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SV40 NLS-AcrIIA4
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDB_270

   Plasmid

   #231111

   Purpose

   C>T base editor

   Depositor

   David Root, John Doench

   Insert

   TadA-CDd-nCas9-SpG-UGI-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

    Plasmid

    #207180

    Purpose

    Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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