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pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4

Plasmid

#62128

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

CRISPR and Gateway Cloning

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 stuffer sgRNA scaffold L5-L4

Plasmid

#62129

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

CRISPR and Gateway Cloning

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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PMKO-shNC

Plasmid

#64863

Purpose

Expresses a scrambled shRNA

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Insert

Scrambled shRNA

Use

Retroviral

Promoter

u6

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

Plasmid

#201032

Purpose

Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4e-Kv

Use

AAV

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-ProA1-GCaMP6s

Plasmid

#126002

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GCaMP6s

Use

AAV

Promoter

ProA1

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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12_pAAV-ProC2-CatCh-GFP-WPRE

Plasmid

#125938

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC2

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-ProD1-GCaMP6s

Plasmid

#126005

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GCaMP6s

Use

AAV

Promoter

ProD1

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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136_pAAV-ProA1-CatCh-GFP-WPRE

Plasmid

#125886

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA1

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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198_pAAV-ProD1-CatCh-GFP-WPRE

Plasmid

#125977

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProD1

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hFGFR3-K650M-V5

Plasmid

#214909

Purpose

expression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)

Depositor

Pavel Krejčí

Article

Kunova Bosakova et al Proc Natl Acad Sci U S A. 2019 Feb 19. p

Insert

FGFR3 (FGFR3 Human)

Tags

V5/His

Expression

Mammalian

Mutation

K650M substitution

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLT3REVIR_MARK3 2573

Plasmid

#107233

Purpose

shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLT3REVIR_MARK3 1122

Plasmid

#107232

Purpose

shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain

Plasmid

#228643

Purpose

Bacterial expression of the SARS-CoV-2 nsp3 macrodomain for crystallization of SARS-CoV-2 nsp3 macrodomain in P 4 3

Depositor

ASAP Discovery, Frank von Delft

Insert

SARS-CoV-2 NSP3 macrodomain (ORF1ab SARS-CoV-2)

Tags

His-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTwist-msEEA1

Plasmid

#214991

Purpose

Gateway entry vector with mouse EEA1

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (Eea1 Mouse)

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Gamma

Plasmid

#177963

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Gamma: L13F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Gamma: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) (S )

Expression

Mammalian

Mutation

L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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AiP2105 - pAAV-AiE2638m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230551

Purpose

AiE2638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12045-pAAV-3xSP10ins-AiE0359h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2045)

Plasmid

#163504

Purpose

AiE0359h is an enhancer sequence designed to drive AAV-mediated transgene expression in Pvalb+ cortical interneurons and cerebellar Purkinje cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

MinRho

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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OA-961B

Plasmid

#104967

Purpose

D. suzukii Medea Construct

Depositor

Omar Akbari

Article

Buchman et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Inserts

GFP
dsRed
HR5Ie1

Expression

Insect

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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p4919

Plasmid

#239357

Purpose

Expression of Rice Cullin, Rice SKP1, Rice Rbx1, and G418 resistance by read through transcription after integration into the tubulin locus

Depositor

Mark Carrington

Article

Alexandra et al bioRxiv 2025.06.17.660065

Insert

Rice CUL1, Rice SKP1, Rice RBX1 and G418 resistance

Tags

CUL1, SKP1 and RBX1 are all Ty epitope tagged

Promoter

none

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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p5179

Plasmid

#239358

Purpose

Exrpession of Rice TIR1 F74->G, Rice ARF fragment and blaticidin resistance by read through transaction after integration int the PFR1 locus

Depositor

Mark Carrington

Article

Alexandra et al bioRxiv 2025.06.17.660065

Insert

Rice TIR1, Rice ARF16 and blasticidin resistance

Use

Bacterial

Tags

TIR1 and ARF are both Ty-epitope tagged

Mutation

TIR1 F74->G. ARF16 is residues 784 to 959 only

Promoter

none

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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