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pDB-hPER2-SNAP-CD4-bla

Plasmid

#179452

Purpose

Donor vector to knock in SNAPtag C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

Plasmid

#180811

Purpose

Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His10-BTLAicd (AA190-289)-TwinStrep

Plasmid

#180805

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289)-TwinStrep (BTLA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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CEA-N domain residues 1-132 (CEACAM5)-pET30b

Plasmid

#174693

Purpose

Plasmid expressing CEA-N domain

Depositor

Jean Gariepy

Article

Abdul-Wahid et al Int J Cancer. 2016 Aug 15;139(4):841-53.

Insert

CEA-N Domain

Tags

TEV Cleavage Site and hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-CD45

Plasmid

#175943

Purpose

knockout mouse CD45

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

CD45 gRNA (Ptprc Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Gapdh

Plasmid

#175938

Purpose

knockout mouse Gapdh

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Gapdh gRNA (Gapdh Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP

Plasmid

#164132

Purpose

Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

ciCas9

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGK-Ptrf-mKate2

Plasmid

#128765

Purpose

Fluorescent mKate reporter for Ptrf

Depositor

Deepak Srivastava

Article

Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

Insert

Ptrf promoter (Cavin1 Mouse)

Expression

Mammalian

Promoter

Ptrf

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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T7pr-His6-MBP-TEV-Cas9-BirA*

Plasmid

#159993

Purpose

Bacterial expression of Cas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

Cas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-KLF4

Plasmid

#139828

Purpose

doxycycline-inducible expression of Human KLF4 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-Lrrc40-trunc-IRES-BFP

Plasmid

#117268

Purpose

Retroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFP

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Lrrc40 (Lrrc40 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

The insert is truncated - deletion of bp 334-407 …

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRA/PINCO

Plasmid

#108938

Purpose

Retroviral expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

LTR

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRHVPS9/PINCO

Plasmid

#108939

Purpose

Retroviral expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

LTR

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT2:GUS

Plasmid

#108444

Purpose

Arabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT2 promoter (HD2B Mustard Weed)

Tags

GUS

Expression

Plant

Promoter

HDT2

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT2:HDT2-GFP

Plasmid

#108566

Purpose

Arabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT2 (HD2B Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

HDT2

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hGCN5mut

Plasmid

#103828

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5mut (CRY2, KAT2A Human, Mustard Weed)

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hGCN5-NLS

Plasmid

#103827

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5-NLS (CRY2, KAT2A Human, Mustard Weed)

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hGCN5

Plasmid

#103826

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5 (CRY2, KAT2A Human, Mustard Weed)

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hBcl10_#16

Plasmid

#75253

Purpose

retroviral shRNA vector against human BCL10, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against hBcl10 (BCL10 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hBcl10_#2

Plasmid

#75254

Purpose

retroviral shRNA vector against human BCL10, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against hBcl10 (BCL10 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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