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Showing: 9321 - 9340 of 27527 results

  2. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176577
    Purpose
    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Tags
    Hexahistidine tag and mVenus
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176577-plasmid-map-sequence-id-348269

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  9. pBN095

    Plasmid
    #173765
    Purpose
    express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    delete motif 3
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    173765-plasmid-map-sequence-id-344806

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  10. pBN093

    Plasmid
    #167951
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, delete motif 3
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167951-plasmid-map-sequence-id-344776

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  12. pVD2

    Plasmid
    #165857
    Purpose
    universal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening method
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165857-plasmid-map-sequence-id-335645

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  13. pBAD-MtrxRFP2

    Plasmid
    #172323
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172323-plasmid-map-sequence-id-431098

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  14. pJC8

    Plasmid
    #170757
    Purpose
    Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.
    Depositor
    Roberto Dominguez
    Article
    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi
    Type
    Empty backbone
    Tags
    site 1- V5 tag and site 2- FLAG tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170757-plasmid-map-sequence-id-334720

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Showing: 9321 - 9340 of 27527 results