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pNK007p

Plasmid

#156325

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRF002d

Plasmid

#156323

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRF001d

Plasmid

#156322

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pNK110d

Plasmid

#156321

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 YPet CTAG

Plasmid

#153402

Purpose

Yellow fluorescent protein CTAG codon optimised for Arabidopsis thaliana

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

Ypet CTAG

Expression

Plant

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 eYFP CTAG

Plasmid

#153396

Purpose

Yellow fluorescent protein CTAG codon optimised for Arabidopsis thaliana

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

eYFP CTAG

Expression

Plant

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Prham-M.Ocy1ORF8430P-p15A-aadA

Plasmid

#149339

Purpose

expresses M.Ocy1ORF8430P DNA methyltransferase (M.SpeI ortholog) in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.Ocy1ORF8430P

Expression

Bacterial

Promoter

rhamnose

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-ECM33_intron

Plasmid

#127596

Purpose

low-copy URA-selectable plasmid, ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron

Expression

Yeast

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-RPL27B_intron

Plasmid

#127604

Purpose

low-copy URA-selectable plasmid, RPL27B intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

RPL27B intron

Expression

Yeast

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-APS3_intron

Plasmid

#127605

Purpose

low-copy URA-selectable plasmid, APS3 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

APS3 intron

Expression

Yeast

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-HNT2_intron

Plasmid

#127603

Purpose

low-copy URA-selectable plasmid, HNT2 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

HNT2 intron

Expression

Yeast

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVd2-Dr-TrkB

Plasmid

#127955

Purpose

Myr DrTrkA expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-3xSAG-Dr-hel4-TrkB

Expression

Mammalian

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGD318

Plasmid

#129735

Purpose

Contains a myristoylation sequence in the pGGC000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

Myristoylation sequence (MGRRKRKPK-)

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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JB11

Plasmid

#128916

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab18QL-BirA*-HA-MAO (RAB18 Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB98

Plasmid

#128927

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

RalBSA-BirA*-HA-MAO (RALB Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV700

Plasmid

#119017

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV609

Plasmid

#119014

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV688

Plasmid

#119015

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV689

Plasmid

#119016

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCR-Blunt_mWasabi

Plasmid

#112640

Purpose

Plasmid for PCR amplification of mWasabi DNA template for in vitro transcription

Depositor

Ganna Bilousova

Article

McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

Insert

mWasabi

Use

Pcr template

Promoter

T7

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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