We narrowed to 15,159 results for: NTS;
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Plasmid#87256PurposeExpression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
ExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MTS-CA-c-Src-FLAG
Plasmid#44654DepositorInsertv-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
TagsFLAG tag and mitochondria-targeting sequence (MTS…ExpressionMammalianMutationchanged Tyr 530 to PhePromoterCMV promoterAvailable SinceMay 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR EGFR-SLP76-TagBFP
Plasmid#188630PurposeHuman EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensorDepositorInsertEGFR (EGFR Human, Synthetic)
UseLentiviralTagsSLP76-TagBFPExpressionMammalianPromoterSFFVAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G175R)-Strep
Plasmid#196211PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG175RAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G189E)-Strep
Plasmid#196212PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG189EAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC-BAR-YR
Plasmid#61766PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).DepositorInsertsBar gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…PromotertrpC promoterAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-pNFkB::Cre-mRuby3
Plasmid#164137PurposeLentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFaDepositorInsertNFkB promoter-Cre-T2A-mRuby3
UseCRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…ExpressionMammalianPromoterNFkB synthetic promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTE4497
Plasmid#80339Purposemammalian expression FnCpf1 nuclease and Fn crRNADepositorInsertsFn crRNA
FnCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3
Plasmid#106205PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVMutationGltI: A184V + mRuby3 fusionPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX62
Plasmid#46854PurposeTOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCEDepositorAvailable SinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-cDAXX
Plasmid#169729PurposeBacterial expression of N-terminally 6His tagged cDAXXDepositorAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX68
Plasmid#46857PurposeOP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCEDepositorAvailable SinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MTS-KD-c-Src-FLAG
Plasmid#44653DepositorInsertv-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
TagsFLAG tag and mitochondria-targeting sequence (MTS…ExpressionMammalianMutationchanged lysine 298 to methioninePromoterCMV promoterAvailable SinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAC-C Sars-CoV-2 NSP12_P314L
Plasmid#194813PurposeSars-CoV-2 NSP12_P314L in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTwist-SARS-CoV-2 Δ18 B.1.351v3
Plasmid#169464PurposeEncodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )
ExpressionMammalianAvailable SinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)
Plasmid#149277PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorInsertModified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
UseT-dna vectorMutationT2318C (DNA) in PEBV coat protein sequenceAvailable SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 4-no linker
Plasmid#176925Purposeexpress Granulin 4 without linker 5 in mammalian cellsDepositorInsertGranulin 4 (GRN Human)
TagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-K508M
Plasmid#191753PurposeExpression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)DepositorInsertmGFP-FGFR3IIIc
TagsmGFPExpressionMammalianMutationc.1523A>T (p.K508M) + silent mutation c.1515A&…Available SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-plus
Plasmid#126767PurposeExpression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.DepositorInserteSpCas9-plus
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationK848A, R1060A; amino acids 1005-1013 replaced wit…PromoterCBhAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
px330-GAPDH
Plasmid#136940PurposeNHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4DepositorAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only