163,570 results

1. T7-phiC31-nos3'UTR

   Plasmid

   #182192

   Purpose

   in vitro transcription of phiC31 integrase mRNA

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   phiC31 integrase
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 138-dCas9-Dnmt3a

   Plasmid

   #84570

   Purpose

   PiggyBac transposon system construct with dCas9-Dnmt3a

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF1627

   Plasmid

   #143144

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXJ3 (FOXJ3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211840

   Purpose

   Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   HA and mCherry

   Expression

   Plant

   Mutation

   Promoter

   UBIQUITIN10

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pECIA14

   Plasmid

   #47051

   Purpose

   This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

   Depositor

   Chris Garcia

   Article

   Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

   Type

   Empty backbone

   Use

   Secreted expression in drosophila culture

   Tags

   Alkaline Phosphatase (human, placental), Flag, HR…

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. EFS-SpdCas9

   Plasmid

   #210703

   Purpose

   This plasmid express EFS promoter driven human codon optimized SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a core promoter

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPGK-T7/2-CD44v6 (fl)

   Plasmid

   #137816

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R296K in the V6 region [Please see depositor comm…

   Promoter

   PGK

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJR022

   Plasmid

   #198820

   Purpose

   C. elegans optimized version of mScarlet

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::wrmScarlet::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCF140_Gag-SpyCas9-NLS

   Plasmid

   #225959

   Purpose

   CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-Gag-SpyCas9-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-FRT/TO-3xFLAG-REV7

    Plasmid

    #114127

    Purpose

    Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    REV7 (MAD2L2 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available Since

    Aug. 16, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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