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Atg32 [200-341] 1B

Plasmid

#190431

Purpose

Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

Depositor

Michael Ragusa

Article

Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

Insert

Atg32

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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TDRD3:ABC006-C02:C220502

Plasmid

#183982

Purpose

Baculovirus expression for structure determination. May not contain entire coding region of gene.

Depositor

Cheryl Arrowsmith

Insert

TDRD3-anti-TDRD3 FAB (TDRD3 Human)

Use

Tags

anti-TDRD3 FAB HC: N-terminal STII leader peptid…

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

Plasmid

#183069

Purpose

Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

CRISPR

Tags

His10 and MBP

Expression

Bacterial

Mutation

Wild-type

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAP007

Plasmid

#206809

Purpose

pET-6xHis-MBP-TEV-BabTIR-APAZ/BabAgo - Heterologous BabSPARTA expression

Depositor

Daan Swarts

Article

Finocchio et al Nucleic Acids Res. 2024 Feb 28;52(4):201

Insert

BabSPARTA operon (6xHis-MBP-BabTIR-APAZ and BabAgo)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pZG162

Plasmid

#202581

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pZG164

Plasmid

#202583

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pZG163

Plasmid

#202582

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pZG161

Plasmid

#202580

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG160

Plasmid

#202579

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG159

Plasmid

#202578

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9

Plasmid

#193862

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged proteins. Empty backbone for LIC cloning.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Type

Empty backbone

Use

Tags

GFP11, Hisx6, and R9

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

Plasmid

#186274

Purpose

Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas6-RT-Cas1

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Use

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET30 W96A-GLTP

Plasmid

#170734

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

W96A-GLTP (GLTP Human)

Use

Tags

His

Expression

Bacterial

Mutation

W96A

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

Plasmid

#185713

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed W403 and F753 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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bdSUMO-GFP-HiBit

Plasmid

#170989

Purpose

For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-E5TAT-GFP-HiBit

Plasmid

#170993

Purpose

For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-E5TAT-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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