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Showing: 921 - 940 of 1459 results

  1. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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  2. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid
    #178057
    Purpose
    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.
    Depositor
    Edward Boyden
    Article
    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    XRI-FLAG
    Use
    AAV
    Tags
    FLAG-MBP
    Expression
    Mammalian
    Mutation
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Oct. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178057-plasmid-map-sequence-id-349209

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  5. pMal_Abl-PRM 3R

    Plasmid
    #112086
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    Abl-PRM-3R (ABL1 Human)
    Use
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112086-plasmid-map-sequence-id-236089

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  6. pMAL-Abl-PRM 4R

    Plasmid
    #112087
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    Abl-PRM-4R (ABL1 Human)
    Use
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112087-plasmid-map-sequence-id-236084

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  7. pMal-Abl-PRM 5R

    Plasmid
    #112088
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    PRM-5R (ABL1 Human)
    Use
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112088-plasmid-map-sequence-id-236080

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  9. pMAL-AGR2

    Plasmid
    #18097
    Depositor
    Ronny Drapkin
    Insert
    AGR2 (AGR2 Human)
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only

  10. pVP16-tev-PRT1

    Plasmid
    #83405
    Purpose
    bacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83405-plasmid-map-sequence-id-175081

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  13. pVP16-tev-PRT1-C195A

    Plasmid
    #83408
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C195A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83408-plasmid-map-sequence-id-175084

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  14. pVP16-tev-PRT1-C29A

    Plasmid
    #83407
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C29A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83407-plasmid-map-sequence-id-175083

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  15. pVP16-tev-PRT1-V28A

    Plasmid
    #83406
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    V28A substitution
    Promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83406-plasmid-map-sequence-id-175082

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  16. CRBN_ΔHBD

    Plasmid
    #232779
    Purpose
    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain
    Depositor
    Ivan Dikic
    Article
    Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2
    Insert
    Cereblon (CRBN Human)
    Use
    Tags
    MBP-His6-TEV
    Expression
    Bacterial
    Mutation
    CRBN (residues 47 to 193 and 249 to 436) with GNG…
    Promoter
    T7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232779-plasmid-map-sequence-id-462151

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Showing: 921 - 940 of 1459 results