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CaV1.3e[8a,11,31b,Δ32,42a] mut

Plasmid

#26576

Depositor

Diane Lipscombe

Publication

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CACNA1D (Cacna1d Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

Plasmid

#113885

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMU-ERr

Plasmid

#61170

Purpose

Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtWAK2-mCherry-HDEL

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

April 9, 2015

Availability

Academic Institutions and Nonprofits only

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pPTP1BD181A-mCherry

Plasmid

#40270

Depositor

Martin Offterdinger

Publication

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTPN1 (PTPN1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D181A

Promoter

CMV

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EasyFusion Halo-mAID

Plasmid

#112852

Purpose

General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Halo-mAID

Use

Synthetic Biology

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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NKP66 pEGFP-N2 (1176)

Plasmid

#62039

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Publication

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MARCHV (MARCHF5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EasyFusion T2A-H2B-TagBFP

Plasmid

#113086

Purpose

General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-TagBFP

Use

Synthetic Biology

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pclbw-mitoCFP

Plasmid

#58426

Purpose

Mammalian expression of CFP targeted to the mitochondrial matrix

Depositor

David Chan

Publication

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mito-CFP

Use

Retroviral

Expression

Mammalian

Promoter

cmv-bactin

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pmCherry-FKBP12-C1

Plasmid

#67900

Purpose

Expresses a fusion of mCherry with FKBP12 in mammalian cells

Depositor

Dorus Gadella

Publication

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-FKBP12

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX-Skp2

Plasmid

#19946

Depositor

Yue Xiong

Publication

Michel et al Cell Growth Differ. 1998 Jun . 9(6):435-

Insert

S-phase kinase-associated protein 2 (p45) (SKP2 Human)

Tags

GST

Expression

Mammalian

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LAMP1-SBP-GFP

Plasmid

#120172

Purpose

Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lysosomal-associated membrane protein 1 (Lamp1 Rat)

Tags

SBP and eGFP

Expression

Mammalian

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRAIL-R2

Plasmid

#61383

Purpose

Expresses TRAIL receptor 2

Depositor

Francis Chan

Publication

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRAIL receptor 2 (TNFRSF10B Human)

Tags

none

Expression

Mammalian

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pPyCAG-HA-p110-IP

Plasmid

#15691

Depositor

Shinya Yamanaka

Publication

Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Insert

p110 delta (Pik3cd Mouse)

Tags

HA

Expression

Mammalian

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pOlyA(E69A)

Plasmid

#132392

Purpose

Mutant version of OlyA that senses sphingomyelin without or with cholesterol

Depositor

Arun Radhakrishnan

Publication

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A point mutant (E69A)

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, E69A, C94S, S151C

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST14-Avitag-SpyCatcher002

Plasmid

#102828

Purpose

Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

Depositor

Mark Howarth

Publication

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Avitag-SpyCatcher002

Tags

Avitag, GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4

Plasmid

#71713

Purpose

Membrane scaffold protein (MSP), helix 4 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis

Expression

Bacterial

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

Plasmid

#133028

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Golgin84 (GOLGA5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-673

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCh-KIF5B*-strep

Plasmid

#120164

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 2A Citrine

Expression

Mammalian

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Rtn3L-mNeonGreen

Plasmid

#169034

Purpose

Express human Rtn3L in mammalian cells

Depositor

Gia Voeltz

Publication

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rtn3L (RTN3 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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