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pET29b_SpyTag003-L-IGHJ4_4-His

Plasmid

#233337

Purpose

To express nanobody IGHJ4_4 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_4-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-IGHJ5-His

Plasmid

#233338

Purpose

To express nanobody IGHJ5 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ5-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_VHH16-Cys-His

Plasmid

#233324

Purpose

To express nanobody VHH16 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

VHH16-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_VHH21-Cys-His

Plasmid

#233325

Purpose

To express nanobody VHH21 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

VHH21-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_1-Cys-His

Plasmid

#233326

Purpose

To express nanobody IGHJ4_1 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_1-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_2-Cys-His

Plasmid

#233327

Purpose

To express nanobody IGHJ4_2 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_2-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_3-Cys-His

Plasmid

#233328

Purpose

To express nanobody IGHJ4_3 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_3-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_4-Cys-His

Plasmid

#233329

Purpose

To express nanobody IGHJ4_4 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_4-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ5-Cys-His

Plasmid

#233330

Purpose

To express nanobody IGHJ5 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ5-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-Linker-C6-His

Plasmid

#233331

Purpose

To express nanobody C6 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-Linker-C6-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-VHH16-His

Plasmid

#233332

Purpose

To express nanobody VHH16 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-VHH16-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-IGHJ4_1-His

Plasmid

#233334

Purpose

To express nanobody IGHJ4_1 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_1-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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2x GCN4-LgBiT

Plasmid

#192542

Purpose

Expresses receiver cell components of LOTIIS

Depositor

Jennifer Prescher

Article

Ng et al Bioconjug Chem. 2022 Oct 19;33(10):1876-

Insert

2x GCN4-LgBiT

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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p32.pA.NGFR-mSrtA(Ge)-flag.mCMV.hPGK.GFP.wpre[sequenced]

Plasmid

#226729

Purpose

sortase A(GE) in p30

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Insert

flag

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGMCK-P38-luxAB-DAS+4

Plasmid

#27376

Depositor

Dirk Schnappinger

Article

Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

Insert

luxA, luxBct6

Use

Gateway Cloning

Expression

Bacterial

Mutation

N/A

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2 xNexin

Plasmid

#24963

Depositor

Edward De Robertis

Article

Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

Insert

Nexin

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
CD4d3+4-bio

Plasmid

#32402

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Type

Empty backbone

Tags

biotinylation peptide and ratCd4d3+4

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pEVP-9

Plasmid

#254821

Purpose

EV Packaging vector with five ?N repeats and human CLYBL scaffold

Depositor

Connie Cepko

Article

Ma et al Proc Natl Acad Sci U S A. 2026 Apr 14;12

Insert

CLYBL

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEVPC-5

Plasmid

#254820

Purpose

EV Packaging vector with I3-01 nanocage, NS3/4A wild-type protease, and λN

Depositor

Connie Cepko

Article

Ma et al Proc Natl Acad Sci U S A. 2026 Apr 14;12

Insert

I3-01

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

Plasmid

#32627

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Donor + Sce BFP

Use

Lentiviral

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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