We narrowed to 29,899 results for: des.2

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pLKO-perox-mEm

Plasmid

#185597

Purpose

Lentiviral construct for expression of mEmerald targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mEm

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-ChrimsonR::FusionRed::Kv2.1

Plasmid

#102770

Purpose

"Activate neurons with 2p stimulation"

Depositor

Karel Svoboda

Article

Daie et al Nat Neurosci. 2021 Feb;24(2):259-265. do

Insert

ChrimsonR::FusionRed::Kv2.1

Use

AAV

Tags

FusionRed and Kv2.1 traficking domain

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.1

Plasmid

#208302

Purpose

Expresses ABE-RA3.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.1-SpCas9 D10A

Expression

Mammalian

Mutation

E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLVPT-rtTR-KRAB

Plasmid

#11777

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-NL1A

Plasmid

#15227

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with insert A (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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zCD2

Plasmid

#122031

Purpose

Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

Depositor

David Christianson

Article

Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

Insert

Zebrafish HDAC6 Catalytic Domain 2

Tags

His6-MBP tag with cleavable TEV site

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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p221z-CAS9p-t35s

Plasmid

#118385

Purpose

Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p

Use

CRISPR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-L26-Flag

Plasmid

#19972

Depositor

Moshe Oren

Article

Ofir-Rosenfeld et al Mol Cell. 2008 Oct 24. 32(2):180-9.

Insert

Rpl26 (RPL26 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only
pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEYFP-p23

Plasmid

#108225

Purpose

Mammalian expression vector for EYFP fusion to human p23

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

EYFP-p23 (PTGES3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFTK055

Plasmid

#171327

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

rTA2S-M2 (TetR-3xVP16) transcriptional activator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mEos2-H2B-6

Plasmid

#57384

Purpose

Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

H2B (H2BC11 Human)

Tags

mEos2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pECFP-DEVG-Venus

Plasmid

#24538

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVG-Venus

Tags

ECFP and Venus

Expression

Mammalian

Mutation

DEVDR mutated to DEVG

Promoter

CMV

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pJBL7005

Plasmid

#136944

Purpose

Malachite green aptamer with stability hairpin, T7 promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

Malachite green aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Caspase-1 (C285A)

Plasmid

#131353

Purpose

Expresses Caspase-1 C285A

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

Caspase-1 (C285A) (Casp1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Caspase-1 (C285A)

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMIK-Neo-mYes-Y535F

Plasmid

#18068

Depositor

Marius Sudol

Article

Espanel et al J Biol Chem. 2001 Apr 27. 276(17):14514-

Insert

yes kinase (Yes1 Mouse)

Expression

Mammalian

Mutation

Tyrosine 535 changed to Phenylalanine

Available Since

July 10, 2008

Availability

Academic Institutions and Nonprofits only
pCMVMagFRET-1-NLS

Plasmid

#50748

Purpose

Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-1 with NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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PNMT

Plasmid

#64078

Purpose

Expression of human Phenylethanolamine N-methyltransferase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346

Insert

Phenylethanolamine N-methyltransferase (PNMT Human, Synthetic)

Tags

Histidine tag

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only
p221z-dCAS9p-t35s

Plasmid

#118387

Purpose

Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

dCAS9p

Use

CRISPR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pFG036 + pFG051

Plasmid

#137997

Purpose

Transposon mutagenesis plasmid and the cI repressor expression plasmid

Depositor

Sébastien Rodrigue

Article

Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

Insert

Tn5 tnp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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