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EcpH-TM

Plasmid

#85389

Purpose

Expresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 1) in mammalian cells. Incorporates into viral membrane to label pseudoviruses

Depositor

Gregory Melikyan

Article

Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

Insert

ecliptic pHluorin (EcpH) fused to N-terminus of human ICAM-1 transmembrane domain

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMOS019: ClopHensorN chloride sensor (cytosolic)

Plasmid

#163048

Purpose

ClopHensorN chloride sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ClopHensorN

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRS423-CUP1-His-Ubi

Plasmid

#99541

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-jGCaMP7b-XN

Plasmid

#178362

Purpose

Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.

Depositor

Xiaodong Liu

Article

Geng et al Elife. 2022 Oct 5;11:e76691. doi: 10.755

Insert

jGCaMP7b-XN

Expression

Mammalian

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) FLIPQTV3.0 8m

Plasmid

#63732

Purpose

FLIPQTV3.0 glutamine sensor with R75MY86A mutations

Depositor

Sakiko Okumoto

Article

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 8mM affinity

Expression

Mammalian

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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BtARR3 short (1-392)

Plasmid

#236272

Purpose

Bacterial expression of bovine arrestin-3 short (1-392) L393Stop

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-3 (ARR3 Bovine)

Expression

Bacterial

Mutation

short, (1-392) L393Stop

Promoter

lac

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR3 long

Plasmid

#236266

Purpose

Bacterial expression of bovine arrestin-3 long isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-3 (ARR3 Bovine)

Expression

Bacterial

Mutation

long isoform

Promoter

lac

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR2 long

Plasmid

#236268

Purpose

Bacterial expression of bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-2 (ARRB2 Bovine)

Expression

Bacterial

Mutation

long isoform

Promoter

lac

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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MTK2_023

Plasmid

#123715

Purpose

Encodes pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PcDNA3.1-2Strep

Plasmid

#210343

Purpose

Empty Vector for molecular cloning

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

2Strep tag

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pUCHR-inLuc

Plasmid

#58955

Purpose

HIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-mScarlet

Plasmid

#140875

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFBP_promoter_acceptor

Plasmid

#189920

Purpose

Binary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformation

Depositor

Adam Steinbrenner

Article

Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

Type

Empty backbone

Expression

Plant

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBad-UGAcS

Plasmid

#172137

Purpose

bacteria expression plasmid for a UDP-GlcNAc sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UGAcS

Expression

Bacterial

Mutation

H605Q- please see depositor comments

Promoter

araBAD

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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p155 pCMV-EGFP/beta-gal

Plasmid

#8387

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox; Beta-gal

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-sfGFP-deadTET1CD

Plasmid

#184442

Purpose

Catalytically DEAD TET1CD with scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

Catalytically DEAD TET1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pYZ1389

Plasmid

#215348

Purpose

Nla CRISPRi Delivery Vector

Depositor

Hank Seifert

Article

Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

Insert

Nla CRISPRi Genes (Cas5, Cas8c, Cas7)

Use

CRISPR; Nla i delivery vector

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJH2930

Plasmid

#175283

Purpose

Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Expression

Worm

Promoter

Pges-1

Available Since

Dec. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEX-A-U6-gRNA

Plasmid

#65626

Purpose

Empty vector for the expression U6 driven gRNA

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

U6-gRNA

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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