We narrowed to 10,053 results for: ADA

1. pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR

   Plasmid

   #189943

   Purpose

   Lentiviral vector to co-express an spsgRNA with NLS-tagBFP

   Depositor

   Kenneth Yamada

   Article

   Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7-TetO-GFP_ColE1

   Plasmid

   #173193

   Purpose

   Expresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   T7-TetO
   

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. QUAS-0-T7-TetO-GFP_ColE1

   Plasmid

   #171662

   Purpose

   QUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-T7-TetO-GFP ssrA-T7 Stop
   

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2379-Tier1-PhCMV-PEST

   Plasmid

   #169484

   Purpose

   Tier-1 vector encoding PhCMV-driven PEST tag for destabilization of proteins (PhCMV-PEST-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven PEST degradation tag
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MYMH 552

   Plasmid

   #138082

   Purpose

   Degradation tag variant at C-terminal of mCherry

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   J23104-mCherry-DAS- pSEVA-331Bb
   

   Expression

   Bacterial

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MYMH 551

   Plasmid

   #138081

   Purpose

   Degradation tag variant at C-terminal of mCherry

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   J23104-mCherry-AAV- pSEVA-331Bb
   

   Expression

   Bacterial

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MYMH 550

   Plasmid

   #138080

   Purpose

   Degradation tag variant at C-terminal of mCherry

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   J23104-mCherry-LVA- pSEVA-331Bb
   

   Expression

   Bacterial

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MYMH 549

   Plasmid

   #138079

   Purpose

   Degradation tag variant at C-terminal of mCherry

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   J23104-mCherry-LAA- pSEVA-331Bb
   

   Expression

   Bacterial

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p667-UBC-BRSK2-V5-miniTurbo_IDG-K

   Plasmid

   #135252

   Purpose

   Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   BRSK2-V5-miniTurbo (BRSK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-miniTurbo-V5-BRSK2_IDG-K

    Plasmid

    #135271

    Purpose

    Gateway destination clone of BRSK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-BRSK2 (BRSK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p667-UBC-BRSK2-V5_IDG-K

    Plasmid

    #135254

    Purpose

    Gateway destination clone of BRSK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    BRSK2-V5 (BRSK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEAST_J23101-HipO

    Plasmid

    #128133

    Purpose

    The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    HipO
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLI_C-tag TNF

    Plasmid

    #171178

    Purpose

    inducible expression of C-tag TNF

    Depositor

    Veit Hornung

    Article

    Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

    Insert

    C-tag TNF
    

    Use

    Lentiviral

    Tags

    C-tag (internal)

    Expression

    Mammalian

    Mutation

    ADAM cleavage site of TNF (AA73-76) was exchanged…

    Promoter

    tight TRE promoter

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Plasmid

    #163057

    Purpose

    Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP1-iEER
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

    Plasmid

    #230989

    Purpose

    Co-expressing membrane-localized Voltron2 and soma-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Membrane-localized Voltron2 and soma-localized CheRiff
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57-GFP-2A-MCS

    Plasmid

    #71783

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. 4xHRE-MinTK-CRE-ODD

    Plasmid

    #141147

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    4xHRE-MinTK-CRE-ODD
    

    Use

    Lentiviral

    Mutation

    contains an altered Cre gene modified by the addi…

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLS-SceI

    Plasmid

    #137725

    Purpose

    Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

    Depositor

    Nadav Ahituv

    Article

    Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57-MCS1-P2A-MCS2 (Hygro)

    Plasmid

    #80922

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. iSpyMac

    Plasmid

    #155014

    Purpose

    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

    Insert

    iSpyMac
    

    Expression

    Mammalian

    Mutation

    PID grafted onto SpyCas9 N-terminal domain, R221K…

    Promoter

    EF1ɑ

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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