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We narrowed to 15,909 results for: genome

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Showing: 9421 - 9440 of 15909 results

  2. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid
    #62373
    Purpose
    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells
    Depositor
    David Liu
    Article
    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do
    Insert
    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    Tags
    (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…
    Expression
    Bacterial
    Mutation
    Y67S amino acid substitution in (-30)GFP renders …
    Promoter
    T7
    Available Since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    62373-plasmid-map-sequence-id-263816

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  5. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139996-plasmid-map-sequence-id-275405

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  7. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51760-plasmid-map-sequence-id-392672

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  9. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available Since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  10. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid
    #208944
    Purpose
    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208944-plasmid-map-sequence-id-415672

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  12. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51761-plasmid-map-sequence-id-79377

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  13. p1.1-mCherry

    Plasmid
    #162772
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    red fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162772-plasmid-map-sequence-id-339102

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  14. p1.2-GS-eGFP

    Plasmid
    #162771
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162771-plasmid-map-sequence-id-339094

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  19. pSLQ10878

    Plasmid
    #183964
    Purpose
    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183964-plasmid-map-sequence-id-363120

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Showing: 9421 - 9440 of 15909 results