We narrowed to 20,084 results for: Sele

Showing: 9441 - 9460 of 20084 results

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pcDNA3.1-CRISPR-Puro

Plasmid

#208646

Purpose

pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

Depositor

Quanfu Ma

Type

Empty backbone

Use

CRISPR

Tags

Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

Expression

Mammalian

Promoter

CMV and U6 in tandem

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-Nb37

Plasmid

#205515

Purpose

Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

Depositor

Jin Zhang

Publication

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-Nb37

Tags

Nuclear localization signal (NLS) and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD5

Plasmid

#195843

Purpose

Expresses human FZD5 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Publication

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD5 (FZD5 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HiBiT-FZD3

Plasmid

#195841

Purpose

Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

Depositor

Gunnar Schulte

Publication

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD3 (FZD3 Human)

Tags

HiBiT

Expression

Mammalian

Mutation

I292V and V495I

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyG1_nat

Plasmid

#184911

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_2FLAG-AKAP79ΔPKA

Plasmid

#183452

Purpose

Expression of human AKAP79 lacking the PKA binding site (Δ391-400)

Depositor

Matthew Gold

Publication

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

aa391-400 deleted

Promoter

CMV

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_2FLAG-AKAP79ΔCaM

Plasmid

#183453

Purpose

Expression of human AKAP79 lacking the calmodulin binding site (Δ79-86)

Depositor

Matthew Gold

Publication

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

aa79-86 deleted

Promoter

CMV

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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FAM19A2-V5

Plasmid

#184338

Purpose

Expression of V5-tagged mouse FAM19A2

Depositor

Peter Scheiffele

Publication

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

FAM19A2 (Tafa2 Mouse)

Tags

V5-tag

Expression

Mammalian

Promoter

CMV

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-TARG1

Plasmid

#172586

Purpose

For transient expression of N-terminal GFP-tagged TARG1

Depositor

Karla Feijs

Publication

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EGFP(W58stop)-HiBiT

Plasmid

#180508

Purpose

Mammalian expression of EGFP(W58stop)-HiBiT

Depositor

Masaharu Somiya

Publication

Somiya et al bioRxiv 2022.01.25.477620

Insert

EGFP(W58stop)

Tags

HiBiT

Expression

Mammalian

Mutation

W58(UGG) to amber stop codon(UAG)

Promoter

CMV

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mito-EYFP

Plasmid

#174182

Purpose

Express EYFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EYFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AktAR2

Plasmid

#175013

Purpose

Plasma membrane-targeted AktAR2.

Depositor

Jin Zhang

Publication

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

Lyn-mCherry-Akt-STOPS

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND2

Plasmid

#172629

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmRuby2-CrowdingLinker-Clover

Plasmid

#171058

Purpose

Expresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.

Depositor

Ke Xu

Publication

Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

Insert

Crowdedness-sensitive linker domain

Tags

mClover2 and mRuby2

Expression

Mammalian

Promoter

CMV

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTAPmyc-T2A-tagRFP

Plasmid

#140278

Purpose

Backbone for making a mammalian expression vector of the protein of interest

Depositor

Hirohide Saito

Publication

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Empty backbone

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98 IRES EGFP

Plasmid

#130706

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hE2F7mg

Plasmid

#73073

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette)

Depositor

Stefan Stamm

Publication

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-C-CARMIL2b

Plasmid

#118741

Purpose

To express CARMIL2 with GFP tag on N-terminus

Depositor

John Cooper

Publication

Lanier et al Mol Biol Cell. 2015 Dec 15;26(25):4577-8

Insert

CARMIL2 (CARMIL2 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-GEM

Plasmid

#111191

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Publication

Piddini et al EMBO J. 2001 Aug 1. 20(15):4076-87.

Insert

GEM (GEM Human)

Tags

EGFP

Expression

Mammalian

Mutation

T2A

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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hHSF1 K298R

Plasmid

#118362

Purpose

This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

Depositor

Lea Sistonen

Publication

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K298R

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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