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MTK1_025

Plasmid

#123687

Purpose

Encodes ConL3 and human D1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L3-D1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_026

Plasmid

#123688

Purpose

Encodes ConL4 and human E1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-E1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_U2AF1_RRM

Plasmid

#110033

Purpose

Protein expression and purification of U2AF1_RRM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

U2AF1_RRM (U2AF1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NXT1_NTF2

Plasmid

#109803

Purpose

Protein expression and purification of NXT1_NTF2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NXT1_NTF2 (NXT1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab3aa

Plasmid

#109703

Purpose

Multisite gateway vector for 3' tagging with rab3aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab3aa (rab3aa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Dendra2-Zyxin-6

Plasmid

#57750

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Zyxin (ZYX Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pENTRdeltaGFPmirGE-DroshaT1.5.5

Plasmid

#61084

Purpose

pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

Depositor

Patrick Salmon

Article

Myburgh et al Mol Ther Nucleic Acids. 2014 Oct 28;3:e2

Insert

deltaGFP

Use

RNAi

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2_BirA

Plasmid

#136521

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA

Use

Entry vector for gateway cloning

Mutation

R118G (highly promiscuous form)

Available Since

June 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest53-SHP-1 S591D

Plasmid

#21803

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 591 to Aspartic acid

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
pHH0103_VCL_Vinculin

Plasmid

#110045

Purpose

Protein expression and purification of VCL_Vinculin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VCL_Vinculin (VCL Human)

Expression

Bacterial

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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p3039 pVL E6-AP

Plasmid

#8646

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Insect

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pTrichoGate-10

Plasmid

#160710

Purpose

For C-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic mCherry

Use

Synthetic Biology

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-8

Plasmid

#160708

Purpose

For N-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-7

Plasmid

#160707

Purpose

For N-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic mCherry

Use

Synthetic Biology

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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p068 pCS mTRPS1 (M)

Plasmid

#11040

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 mut (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Two Cys residues at positions 917 and 920 in the …

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
MTK8a_005

Plasmid

#123889

Purpose

Encodes the F1 Kanamycin resistance cassette as a type 8a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-KanR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK7_012

Plasmid

#123879

Purpose

Encodes the 5' 500 bp hCYBYL locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hCLYBL

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8b_002

Plasmid

#123891

Purpose

Encodes the 3' 500 bp hRosa26 locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_hROSA26

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK8b_009

Plasmid

#123896

Purpose

Encodes the 3' 500 bp hThal5.10-2-hg18 locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_hThal5.10-2-hg18

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK8b_010

Plasmid

#123897

Purpose

Encodes the 3' 500 bp hCLYBL locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_hCLYBL

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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