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pcDNA4-PKCZeta WT His tagged

Plasmid

#24609

Depositor

Jeff Wrana

Article

Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Insert

PKCZeta (PRKCZ Human)

Tags

His

Expression

Mammalian

Available Since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
QUAS-5-T7-GFP_ColE1

Plasmid

#171653

Purpose

QUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-5-T7

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-MSCV-4xS1m-GFP-T2A-PU

Plasmid

#138581

Purpose

For in vivo tagging

Depositor

Yin-Yuan Mo

Article

Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

Type

Empty backbone

Use

Lentiviral

Tags

S1m tag

Promoter

MSCV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-EnRRbpj

Plasmid

#160925

Purpose

dominant-negative construct to inhibit Notch signaling

Depositor

Mark Emerson

Article

Chen et al Sci Rep. 2021 Jul 15;11(1):14525. doi: 1

Insert

EnRRbpj

Mutation

fused with an engrailed repressor

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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PKC delta CAT

Plasmid

#16388

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta (Prkcd Mouse)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, aa 334-674

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pBbB5c-GFP

Plasmid

#35353

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-nMyc-wt_3'UTR

Plasmid

#31858

Depositor

Robert Blelloch

Article

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

nMyc wild-type 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Plk1 FRET sensor c-jun substrate

Plasmid

#45203

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Plk1 FRET sensor with c-jun substrate

Tags

CyPET, YPET

Expression

Mammalian

Promoter

T7

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pBbE2a-RFP

Plasmid

#35322

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCVII-E2Crimson

Plasmid

#162756

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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FRP467-PACT1(-1-520)-LexA-ER-haVP16

Plasmid

#58430

Purpose

Expresses LexA-ER-VP16 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-VP16

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pJBL3752

Plasmid

#128809

Purpose

Fluoride riboswitch-regulated sfGFP

Depositor

Julius Lucks

Article

Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

Insert

Fluoride riboswitch-regulated sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-TAP

Plasmid

#12570

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Type

Empty backbone

Use

Retroviral

Tags

TAP

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pMSCVII-mChe

Plasmid

#162753

Purpose

Retroviral (MSCV) expression of mCherry fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mCherry

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMEXNH3

Plasmid

#110079

Purpose

Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

His10 Tag - 3C cleavage site

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eGFP-LIC

Plasmid

#40768

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Type

Empty backbone

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pJJS012

Plasmid

#188335

Purpose

PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::mTagBFP2::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/B0,+

Plasmid

#112798

Purpose

Expression of ATB0,+

Depositor

Katarzyna Nalęcz

Article

Samluk et al Biochem Biophys Res Commun. 2012 May 25;

Insert

B(0,+)/SLC6A14 (Slc6a14 Rat)

Tags

3 x Flag

Expression

Mammalian

Mutation

L350S

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pKL11-GCaMP6f bb100

Plasmid

#158983

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23100 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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