We narrowed to 14,449 results for: RING

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pRRL-VinculinVenus-S1033A

Plasmid

#211896

Purpose

Vinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

Depositor

Brenton Hoffman

Article

Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

Insert

VinculinVenus-S1033A (VCL Chicken)

Use

Lentiviral

Tags

VenusA206K

Mutation

mutated vinculin serine 1033 to alanine (S1033A),…

Promoter

CMV

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinVenus-S1033D

Plasmid

#211897

Purpose

Vinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

Depositor

Brenton Hoffman

Article

Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

Insert

VinculinVenus-S1033D (VCL Chicken)

Use

Lentiviral

Tags

VenusA206K

Mutation

mutated vinculin serine 1033 to aspartic acid (S1…

Promoter

CMV

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pv6_dPC_Chromo_TAF3_Phd

Plasmid

#179403

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

Plasmid

#101205

Purpose

Bacterial expression plasmid for SpCas9 REC3 domain

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K506–Q712

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K506–Q712

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBTK601

Plasmid

#110607

Purpose

BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Cas9

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

Plasmid

#136381

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV-T2A-MCP-TV

Use

CRISPR

Expression

Plant

Mutation

D570A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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SNAP-MIDAS

Plasmid

#171604

Purpose

Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)

Tags

His6 tag and SNAP tag

Expression

Bacterial

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFBP-4_1.sensor

Plasmid

#162844

Purpose

Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

4_1_RNA-device

Expression

Yeast

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFBP-4_1m.mutant

Plasmid

#162869

Purpose

Yeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

4_1m_RNA-device

Expression

Yeast

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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Cs2+Etv4(pea3)

Plasmid

#49093

Purpose

expression of Etv4

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv4 (etv4 Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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LB334 (Bcl-2 from -1287 to -1640)

Plasmid

#15241

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -1640 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)_No_FLAG_ATP1A1-G7

Plasmid

#173203

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159870

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Actin.CIB.GFP

Plasmid

#133618

Purpose

Expresses beta actin - CIBN - GFP protein fusion

Depositor

Robert Hughes

Article

Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

Insert

betaActin - CIB

Tags

GFP

Expression

Mammalian

Promoter

cmv

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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CFP-PBD(H83,86L)

Plasmid

#11402

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

p21-activated kinase 1 (PAK1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Obtained with Histidine residues 83 and 86 change…

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
pSR357.9

Plasmid

#125105

Purpose

Expression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)129
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159867

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD

Plasmid

#25994

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Plasmid

#159861

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_B2121

Plasmid

#91065

Purpose

Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Promoter

GmUbi

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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