We narrowed to 10,922 results for: 158

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GFP-KIF1B beta-FL

Plasmid

#79108

Purpose

Mammalian expression of GFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

GFP-KIF1B beta full length (KIF1B Human)

Tags

GFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pDY0207 ACTB atgRNA with v1 scaffold

Plasmid

#179107

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA

Use

CRISPR

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDY0216 PASTEv1 PE2-P2A-Bxb1

Plasmid

#179104

Purpose

PASTEv1 PE2-P2A-Bxb1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-PE2-P2A-Bxb1

Expression

Mammalian

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_WT

Plasmid

#81737

Purpose

Gateway Donor vector containing PTEN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKCA_WT

Plasmid

#82225

Purpose

Gateway Donor vector containing PRKCA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKCA (PRKCA Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMXS-mitoGshF-blasticidin

Plasmid

#210314

Purpose

Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells

Depositor

Kivanc Birsoy

Article

Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

Insert

Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)

Use

Retroviral

Tags

FLAG peptide, Homo sapiens ACO2 mitochondrial tar…

Mutation

Codon optimized for expression in human cells

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_VHL_WT

Plasmid

#81874

Purpose

Gateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJT331 Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast

Plasmid

#195538

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDY0310 TP901-1 Integrase

Plasmid

#179110

Purpose

TP901-1 Integrase expression vector

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

TP901-1 Integrase

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcs2-ATF4uORF1-2-GFP-IRES-mCherry

Plasmid

#226105

Purpose

ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

atf4 (ATF4 Human)

Tags

GFP

Expression

Mammalian

Mutation

contains uORF1-2 of ATF4, whose stability can ser…

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DL5A-Fc(DAPA)-AviTag-6xHis

Plasmid

#156643

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL5A (KIR2DL5A Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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10xHis-3C-BRD4(42-459) BD1-BD2

Plasmid

#233886

Purpose

pRSF-DUET1 for protein overexpression of human BRD4

Depositor

Alessio Ciulli

Article

Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

Insert

BRD4 (BRD4 Human)

Tags

10x his 3C

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLX_314-HRI-V5

Plasmid

#202434

Purpose

Expression of HRI

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

HRI (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AMBRA1-3xFLAG

Plasmid

#172605

Purpose

Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-GsCT

Plasmid

#205516

Purpose

Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-GsCT (GNAS Human, Synthetic)

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF3_WT

Plasmid

#81755

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SUV39H1_WT

Plasmid

#82236

Purpose

Gateway Donor vector containing SUV39H1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SUV39H1 (SUV39H1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP in Artichoke

Plasmid

#169931

Purpose

Overexpression of Full Length of BCL6-GFP fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 (BCL6 Human)

Use

Lentiviral

Tags

GFP

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_TMPO_WT

Plasmid

#81818

Purpose

Gateway Donor vector containing TMPO , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TMPO (TMPO Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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