We narrowed to 10,121 results for: EPO
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Plasmid#159088PurposeLR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA insertsDepositorInsertsUseFluorescent reporterPromoter1.7kb fabp6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
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pGL3 2198
Plasmid#49347PurposeThis is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.DepositorInsertBmp2 promoter (shorter sequence) (BMP2 Human)
UseLuciferaseTagsluciferasePromoterBmp2 promoterAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
p1.1-mCherry
Plasmid#162772PurposeFluorescent reporter for CHO expression studiesDepositorInsertred fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1.2-GS-eGFP
Plasmid#162771PurposeFluorescent reporter for CHO expression studiesDepositorInsertenhanced green fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-E112A-FL
Plasmid#110218PurposeExpression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsHis6 and ZBExpressionBacterialMutationE112A, P296L (see depositor comments below)Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tre-Tight ifgMosaic Donor vector (LH121)
Plasmid#99630PurposeTo clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/LoxMutation(Please see depositor comments below)Available SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMiR-RECK-3'UTR-miR-21-mut
Plasmid#53693PurposeLuciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding siteDepositorInsertRECK-3'UTR (RECK Human)
UseLuciferaseMutationnucleotide sequence 1083-1086 is mutated to TTCGAvailable SinceJuly 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL2-hOct4 DE-SV40-Luc
Plasmid#26279PurposeAddgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.DepositorInsertOct4 Distal Enhancer Element (DE) (POU5F1 Human)
UseLuciferaseExpressionMammalianMutationSee Depositor Comments belowAvailable SinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIS1 INSIG2
Plasmid#60798PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and wild-type miR-155 sitesDepositorInsertINSIG2 3'UTR and wild-type miR-155 binding site (INSIG2 Human)
UseLuciferaseExpressionMammalianPromoterthymidine kinase (HSV-TK promoter)Available SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry
Plasmid#179429PurposeDual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-5kb-mch
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(FF)
Plasmid#27135DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationTyrosines in ITAMS 1-3 mutated to phenylalanines …Available SinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1
Plasmid#173947PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 from SOX9 enhancer cluster EC1.45
UseLuciferaseMutationNonePromoterSV40 promoterAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-20a-3p
Plasmid#103341PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-20a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-20a-3p target (MIR20A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIS1 mPELO
Plasmid#60805PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains PELO 3' UTR and mutated miR-155 sitesDepositorInsertPELO 3'UTR and mutated miR-155 binding site (PELO Human)
UseLuciferaseExpressionMammalianMutationmutated miR-155 binding sitePromoterthymidine kinase (HSV-TK promoter)Available SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIS1 mATP2B1
Plasmid#60789PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains ATP2B1 3' UTR and mutated miR-155 sitesDepositorInsertATP2B1 3'UTR and mutated miR-155 binding site (ATP2B1 Human)
UseLuciferaseExpressionMammalianMutationmutated miR-155 binding sitePromoterthymidine kinase (HSV-TK promoter)Available SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-Y117C
Plasmid#110216PurposeExpression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsFlagExpressionMammalianMutationY117C, P296L (see depositor comments below)Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Enhancer-ZC3H12D_PromoterMUT
Plasmid#153068PurposeZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporterDepositorInsertZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
UseLuciferaseExpressionMammalianMutationMutagenesis to disrupt 3 BHLHE40 binding sites. N…PromoterNoneAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-mouseEC1.45
Plasmid#173972PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertMouse orthologous SOX9 enhancer cluster EC1.45
UseLuciferaseMutationNonePromoterSV40 promoterAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
LIC4B-His-TEV-TAK1-TAB1
Plasmid#154874PurposeInsect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91DepositorInsertTAK1-TAB1 (TAB1 Human)
TagsLIC TEV HisExpressionInsectMutationCodon optimizedPromoterpolyhedrinAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIS1 mINSIG2
Plasmid#60799PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and mutated miR-155 sitesDepositorInsertINSIG2 3'UTR and mutated miR-155 binding site (INSIG2 Human)
UseLuciferaseExpressionMammalianMutationmutated miR-155 binding sitePromoterthymidine kinase (HSV-TK promoter)Available SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only