179,333 results
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Plasmid#8822DepositorAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only
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4787 TRV2-eTnpBc-HHreRNA4PDS
Plasmid#251817PurposeEditing Photene desaturase (PDS) genes in Nicotiana benthamianaDepositorInsertEnhanced variant of ISDra2 eTnpBc with guide targeting NbPDS flanked by HH-HDV ribozymes
TagsAtFDnlsExpressionPlantMutationN4Y/R110K/V192L/L222IAvailable sinceMarch 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pET21b FAST-PETase
Plasmid#225760PurposeExpresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tagDepositorInsertFAST-PETase
Tags6xHis TagExpressionBacterialPromoterT7Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-Puro (pRW1441)
Plasmid#216217PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SPLICS LY-MT Long P2A
Plasmid#213613PurposeDetect the long-range Lysosome-Mitochondria contactDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSPLICS LY-MT Long P2A
ExpressionMammalianAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-nLuc
Plasmid#212611PurposeFor expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTornado-CVB3-nLuc
UseLuciferaseExpressionMammalianPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Control Vector
Plasmid#212937PurposeControl vector with constitutive firefly luciferase expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertluc+
UseLuciferaseMutationWTPromoterSV40Available sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pHCMV-VSVgSS-pAG-TM
Plasmid#207318PurposeFunctional incorporation of protein AG (pAG) fusion protein into envelope of virionsDepositorInsertprotein AG
ExpressionMammalianPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-R-iLACCO1.2
Plasmid#208027PurposeBiosensor for intracellular L-lactateDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertR-iLACCO1.2
ExpressionMammalianAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-iCasp9
Plasmid#199441PurposeExpress inducible Caspase9 in mammalian cellsDepositorInsertinducible Caspase9 (CASP9 Human)
UseLentiviralTagsDmrB and mCherryExpressionBacterial and MammalianAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-luc2
Plasmid#194384PurposeReporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert6xCRE-CMVmin-luc2
ExpressionMammalianPromoterCMV minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpCas9 TadCBEd-V106W
Plasmid#193839PurposeExpress TadCBEd-V106W (with SpCas9) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTadA-CDd V106W-SpCas9 D10A-2xUGI
ExpressionMammalianAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-EGFP-caax
Plasmid#190155PurposeExpresses membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorHas serviceAAV1 and AAV8InsertEGFP-caax
UseAAVExpressionMammalianPromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)
Plasmid#185911PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized ABE8e-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3/er-(n2)oxStayGold(c4)
Plasmid#185822PurposeA soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserter-(n2)oxStayGold(c4)
ExpressionMammalianPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide-puro-NLS-GFP
Plasmid#185473PurposeMammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMammalianPromoterCMVAvailable sinceJuly 1, 2022AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
SpySwitch
Plasmid#184225PurposeExpresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.DepositorInsertSpySwitch
TagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GalT-RpHLuorin2
Plasmid#171719PurposeTrans-Golgi expression of ratiometric pHLuorin2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRatiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pETDuet S2
Plasmid#171921PurposeExpress RNA-based (p)ppGpp sensor and a NirFPDepositorInsertsRNA-based (p)ppGpp sensor
NirFP
ExpressionBacterialPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Go1-CASE
Plasmid#168123PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at L91/G92 within GNAO1Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by