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We narrowed to 31,746 results for: son

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Showing: 9561 - 9580 of 31746 results

  1. peSCBE3-NG-Hypa

    Plasmid
    #218165
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218165-plasmid-map-sequence-id-433201

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  2. peGFP C3-SPG20 8xV

    Plasmid
    #218579
    Purpose
    Expresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells
    Depositor
    Hemmo Meyer
    Publication
    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPG20 (SPART Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    changed Tryptophan 419, Leucine 438, Isoleucine 4…
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218579-plasmid-map-sequence-id-432382

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  4. AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1

    Plasmid
    #205444
    Purpose
    human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59
    Depositor
    Deepta Bhattacharya
    Publication
    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iCasp9-HLA-E SCT-hCD55
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    205444-plasmid-map-sequence-id-409031

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  6. Anti-mGluR1/5 (Group I) glutamate receptor [N75/33R]

    Plasmid
    #177570
    Purpose
    Mammalian Expression Plasmid of anti-mGluR1/5 (Group I) glutamate receptor (Rat). Derived from hybridoma N75/33.
    Depositor
    James Trimmer
    Publication
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-mGluR1/5 (Group I) glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grm5 Mouse)
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    177570-plasmid-map-sequence-id-361578

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  7. pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting

    Plasmid
    #156431
    Purpose
    Targeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTG
    Depositor
    Elphege Nora
    Publication
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAD21, Halotag (Rad21 Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    156431-plasmid-map-sequence-id-310238

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  8. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139097-plasmid-map-sequence-id-273896

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  9. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid
    #126425
    Purpose
    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
    Expression
    Mammalian
    Mutation
    alanine-6 of TdT has been changed to a STOP codon
    Promoter
    pCMV
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    126425-plasmid-map-sequence-id-252600

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  12. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

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  13. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

    Plasmid
    #210710
    Purpose
    This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9
    Depositor
    Isaac Hilton
    Publication
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9 (STAT1, MRTFA Synthetic, Human, Staphylococcus aureus)
    Use
    AAV and CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A/N580A
    Promoter
    M11
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210710-plasmid-map-sequence-id-416264

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  14. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

    Plasmid
    #205443
    Purpose
    human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55
    Depositor
    Deepta Bhattacharya
    Publication
    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hCD46-HLA-G SCT-hCD47-hCD59
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    205443-plasmid-map-sequence-id-408942

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  16. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

    Plasmid
    #169185
    Purpose
    Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Publication
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6-3xFlag (nsp12 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169185-plasmid-map-sequence-id-334217

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  18. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

    Plasmid
    #104569
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1(D832A)-DmrA(x2)
    Tags
    3x FLAG, 3x HA, and NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    104569-plasmid-map-sequence-id-201433

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  19. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

    Plasmid
    #104568
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1(D832A)-DmrA(x1)
    Tags
    3x FLAG, 3x HA, and NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    104568-plasmid-map-sequence-id-201431

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  20. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid
    #223079
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223079-plasmid-map-sequence-id-447658

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Showing: 9561 - 9580 of 31746 results